1brl

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[[Image:1brl.jpg|left|200px]]
 
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{{Structure
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==THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION==
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|PDB= 1brl |SIZE=350|CAPTION= <scene name='initialview01'>1brl</scene>, resolution 2.4&Aring;
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<StructureSection load='1brl' size='340' side='right'caption='[[1brl]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1brl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. The June 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Luciferase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_6 10.2210/rcsb_pdb/mom_2006_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BRL FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1brl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1brl OCA], [https://pdbe.org/1brl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1brl RCSB], [https://www.ebi.ac.uk/pdbsum/1brl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1brl ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1brl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1brl OCA], [http://www.ebi.ac.uk/pdbsum/1brl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1brl RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/LUXA_VIBHA LUXA_VIBHA] Light-emitting reaction in luminous bacteria.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/1brl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1brl ConSurf].
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<div style="clear:both"></div>
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'''THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION'''
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==See Also==
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*[[Luciferase 3D structures|Luciferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Luciferases are a class of enzymes that generate light in the visible spectrum. Luciferase from luminous marine bacteria is an alpha-beta heterodimer monooxygenase that catalyzes the oxidation of FMNH2 and a long-chain aliphatic aldehyde. The X-ray crystal structure of bacterial luciferase from Vibrio harveyi has been determined to 2.4 A resolution. The structure was solved by a combination of multiple isomorphous replacement and molecular averaging between the two heterodimers in the asymmetric unit. Each subunit folds into a (beta/alpha)8 barrel motif, and dimerization is mediated through a parallel four-helix bundle centered on a pseudo 2-fold axis that relates the structurally similar subunits. The vicinity of the active site has been identified on the alpha subunit by correlations with similar protein motifs and previous biochemical studies. The structure presented here represents the first molecular model of a bioluminescent enzyme.
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[[Category: Large Structures]]
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==About this Structure==
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1BRL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. The following page contains interesting information on the relation of 1BRL with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRL OCA].
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==Reference==
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Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7756289 7756289]
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[[Category: Alkanal monooxygenase (FMN-linked)]]
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[[Category: Luciferase]]
[[Category: Luciferase]]
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[[Category: Protein complex]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
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[[Category: Fisher, A J.]]
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[[Category: Fisher AJ]]
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[[Category: Rayment, I.]]
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[[Category: Rayment I]]
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[[Category: monooxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:06:46 2008''
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Current revision

THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION

PDB ID 1brl

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