4v17

<StructureSection load='4v17' size='340' side='right' caption='[[4v17]], [[Resolution|resolution]] 2.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[4v17]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V17 FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v18|4v18]], [[4v1b|4v1b]], [[4v1i|4v1i]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v17 OCA], [http://pdbe.org/4v17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v17 RCSB], [http://www.ebi.ac.uk/pdbsum/4v17 PDBsum]</span></td></tr>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

Anaerobic bacteria organize carbohydrate-active enzymes into a multi-component complex, the cellulosome, which degrades cellulose and hemicellulose highly efficiently. Genome sequencing of Ruminococcus flavefaciens FD-1 offers extensive information on the range and diversity of the enzymatic and structural components of the cellulosome. The R. flavefaciens FD-1 genome encodes over 200 dockerin-containing proteins, most of which are of unknown function. One of these modular proteins comprises a glycoside hydrolase family 5 catalytic module (GH5) linked to an unclassified carbohydrate-binding module (CBM-Rf1) and a dockerin. The novel CBM-Rf1 has been purified and crystallized. The crystals belonged to the trigonal space group R32:H. The CBM-Rf1 structure was determined by a multiple-wavelength anomalous dispersion experiment using AutoSol from the PHENIX suite using both selenomethionyl-derivative and native data to resolutions of 2.28 and 2.0 A, respectively.

 

Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.,Venditto I, Centeno MS, Ferreira LM, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1653-6. doi:, 10.1107/S2053230X14024248. Epub 2014 Nov 14. PMID:25484220<ref>PMID:25484220</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<references/>

[[Category: Centeno, M S.J]]

[[Category: Ferreira, L M.A]]

[[Category: Fontes, C M.G A]]

[[Category: Najmudin, S]]

[[Category: Venditto, I]]

[[Category: Sugar binding protein]]