4xga

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:27, 8 November 2023) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of BamB and BamA P3-5 complex from E.coli==
==Crystal structure of BamB and BamA P3-5 complex from E.coli==
-
<StructureSection load='4xga' size='340' side='right' caption='[[4xga]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
+
<StructureSection load='4xga' size='340' side='right'caption='[[4xga]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4xga]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XGA FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4xga]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XGA FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xga OCA], [http://pdbe.org/4xga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xga RCSB], [http://www.ebi.ac.uk/pdbsum/4xga PDBsum]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xga OCA], [https://pdbe.org/4xga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xga RCSB], [https://www.ebi.ac.uk/pdbsum/4xga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xga ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref> [[http://www.uniprot.org/uniprot/BAMA_ECOLI BAMA_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamD, the core component of the assembly machinery.<ref>PMID:15951436</ref> <ref>PMID:16102012</ref> <ref>PMID:16824102</ref> <ref>PMID:20378773</ref> <ref>PMID:21823654</ref>
+
[https://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
In Escherichia coli, the Omp85 protein BamA and four lipoproteins (BamBCDE) constitute the BAM complex, which is essential for the assembly and insertion of outer membrane proteins into the outer membrane. Here, the crystal structure of BamB in complex with the POTRA3-4 domains of BamA is reported at 2.1 A resolution. Based on this structure, the POTRA3 domain is associated with BamB via hydrogen-bonding and hydrophobic interactions. Structural and biochemical analysis revealed that the conserved residues Arg77, Glu127, Glu150, Ser167, Leu192, Leu194 and Arg195 of BamB play an essential role in interaction with the POTRA3 domain.
 +
 
 +
Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA.,Chen Z, Zhan LH, Hou HF, Gao ZQ, Xu JH, Dong C, Dong YH Acta Crystallogr D Struct Biol. 2016 Feb;72(Pt 2):236-44. doi:, 10.1107/S2059798315024729. Epub 2016 Jan 22. PMID:26894671<ref>PMID:26894671</ref>
 +
 
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 4xga" style="background-color:#fffaf0;"></div>
 +
 
 +
==See Also==
 +
*[[Bam complex 3D structures|Bam complex 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Chen, Z]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Dong, C]]
+
[[Category: Large Structures]]
-
[[Category: Dong, Y H]]
+
[[Category: Chen Z]]
-
[[Category: Gao, Z Q]]
+
[[Category: Dong C]]
-
[[Category: Zhan, L H]]
+
[[Category: Dong YH]]
-
[[Category: Outer member protein]]
+
[[Category: Gao ZQ]]
-
[[Category: Protein binding-membrane protein complex]]
+
[[Category: Zhan LH]]

Current revision

Crystal structure of BamB and BamA P3-5 complex from E.coli

PDB ID 4xga

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xga"
Personal tools