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| - | [[Image:1bw7.jpg|left|200px]] | |
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| - | {{Structure
| + | ==SOLUTION STRUCTURE OF A DNA DUPLEX CONTAINING A REPLICABLE DIFLUOROTOLUENE-ADENINE PAIR== |
| - | |PDB= 1bw7 |SIZE=350|CAPTION= <scene name='initialview01'>1bw7</scene>
| + | <StructureSection load='1bw7' size='340' side='right'caption='[[1bw7]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DFT:1-[2-DEOXYRIBOFURANOSYL]-2,4-DIFLUORO-5-METHYL-BENZENE-5'MONOPHOSPHATE'>DFT</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1bw7]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BW7 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DFT:1-[2-DEOXYRIBOFURANOSYL]-2,4-DIFLUORO-5-METHYL-BENZENE-5MONOPHOSPHATE'>DFT</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bw7 OCA], [https://pdbe.org/1bw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bw7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bw7 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bw7 OCA], [http://www.ebi.ac.uk/pdbsum/1bw7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bw7 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''SOLUTION STRUCTURE OF A DNA DUPLEX CONTAINING A REPLICABLE DIFLUOROTOLUENE-ADENINE PAIR'''
| + | [[Category: Guckian KM]] |
| - | | + | [[Category: Kool ET]] |
| - | | + | [[Category: Krugh TR]] |
| - | ==Overview==
| + | |
| - | A nonpolar aromatic nucleoside derivative based on 2,4-difluorotoluene (F), a non-hydrogen bonding shape analog of thymidine, was recently shown to be replicated against adenine with high efficiency and fidelity. This led to the suggestion that geometric matching, potentially even in the absence of hydrogen bonding between bases in a pair, may be sufficient to direct nucleotide selection during replication. We have examined the solution structure of the F-A pair in the context of a 12 base pair DNA duplex. We find that, despite the destabilization caused by this analog, the F-A pair very closely resembles that of a T x A pair in the same context. This lends support to the importance of shape matching in replication.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1BW7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW7 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Solution structure of a DNA duplex containing a replicable difluorotoluene-adenine pair., Guckian KM, Krugh TR, Kool ET, Nat Struct Biol. 1998 Nov;5(11):954-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9808039 9808039]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Guckian, K M.]] | + | |
| - | [[Category: Kool, E T.]] | + | |
| - | [[Category: Krugh, T R.]] | + | |
| - | [[Category: difluorotoluene]]
| + | |
| - | [[Category: dna]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:09:27 2008''
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