5hoe

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'''Unreleased structure'''
 
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The entry 5hoe is ON HOLD
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==Crystal structrue of Est24, a carbohydrate acetylesterase from Sinorhizobium meliloti==
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<StructureSection load='5hoe' size='340' side='right'caption='[[5hoe]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5hoe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti_1021 Sinorhizobium meliloti 1021]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HOE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hoe OCA], [https://pdbe.org/5hoe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hoe RCSB], [https://www.ebi.ac.uk/pdbsum/5hoe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hoe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q92XZ6_RHIME Q92XZ6_RHIME]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Carbohydrate acetylesterases, which have a highly specific role among plant-interacting bacterial species, remove the acetyl groups from plant carbohydrates. Here, we determined the crystal structure of Est24, an octameric carbohydrate acetylesterase from Sinorhizobium meliloti, at 1.45 A resolution and investigated its biochemical properties. The structure of Est24 consisted of five parallel beta strands flanked by alpha helices, which formed an octameric assembly with two distinct interfaces. The deacetylation activity of Est24 and its mutants around the substrate-binding pocket was investigated using several substrates, including glucose pentaacetate and acetyl alginate. Elucidation of the structure-function relationships of Est24 could provide valuable opportunities for biotechnological explorations.
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Authors: Oh, C., Ryu, B.H., An, D.R., Nguyen, D.D., Yoo, W., Kim, T., Ngo, D.T., Kim, H.S., Park, J.S., Kim, K.K., Kim, T.D.
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Structural and Biochemical Characterization of an Octameric Carbohydrate Acetylesterase from Sinorhizobium meliloti.,Oh C, Ryu BH, An DR, Nguyen DD, Yoo W, Kim T, Ngo TD, Kim HS, Kim KK, Kim TD FEBS Lett. 2016 Apr;590(8):1242-52. doi: 10.1002/1873-3468.12135. Epub 2016 Mar, 31. PMID:26991446<ref>PMID:26991446</ref>
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Description: Crystal structrue of Est24, a carbohydrate acetylesterase from Sinorhizobium meliloti
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Ngo, D.T]]
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<div class="pdbe-citations 5hoe" style="background-color:#fffaf0;"></div>
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[[Category: Nguyen, D.D]]
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== References ==
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[[Category: Yoo, W]]
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<references/>
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[[Category: Ryu, B.H]]
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__TOC__
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[[Category: An, D.R]]
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</StructureSection>
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[[Category: Park, J.S]]
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[[Category: Large Structures]]
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[[Category: Kim, T.D]]
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[[Category: Sinorhizobium meliloti 1021]]
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[[Category: Oh, C]]
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[[Category: An DR]]
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[[Category: Kim, T]]
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[[Category: Kim HS]]
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[[Category: Kim, H.S]]
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[[Category: Kim KK]]
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[[Category: Kim, K.K]]
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[[Category: Kim T]]
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[[Category: Kim TD]]
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[[Category: Ngo DT]]
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[[Category: Nguyen DD]]
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[[Category: Oh C]]
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[[Category: Park JS]]
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[[Category: Ryu BH]]
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[[Category: Yoo W]]

Current revision

Crystal structrue of Est24, a carbohydrate acetylesterase from Sinorhizobium meliloti

PDB ID 5hoe

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