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5hoq

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'''Unreleased structure'''
 
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The entry 5hoq is ON HOLD
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==Apo structure of CalS11, TDP-rhamnose 3'-o-methyltransferase, an enzyme in Calicheamicin biosynthesis==
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<StructureSection load='5hoq' size='340' side='right'caption='[[5hoq]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5hoq]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HOQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.793&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hoq OCA], [https://pdbe.org/5hoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hoq RCSB], [https://www.ebi.ac.uk/pdbsum/5hoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hoq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8KNF1_MICEC Q8KNF1_MICEC]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structure analysis and ensemble refinement of the apo-structure of thymidine diphosphate (TDP)-rhamnose 3'-O-methyltransferase reveal a gate for substrate entry and product release. TDP-rhamnose 3'-O-methyltransferase (CalS11) catalyses a 3'-O-methylation of TDP-rhamnose, an intermediate in the biosynthesis of enediyne antitumor antibiotic calicheamicin. CalS11 operates at the sugar nucleotide stage prior to glycosylation step. Here, we present the crystal structure of the apo form of CalS11 at 1.89 A resolution. We propose that the L2 loop functions as a gate facilitating and/or providing specificity for substrate entry or promoting product release. Ensemble refinement analysis slightly improves the crystallographic refinement statistics and furthermore provides a compelling way to visualize the dynamic model of loop L2, supporting the understanding of its proposed role in catalysis.
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Authors: Han, L., Helmich, K.E., Singh, S., Thorson, J.S., Bingman, C.A., Phillips Jr., G.N., Enzyme Discovery for Natural Product Biosynthesis
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Loop dynamics of thymidine diphosphate-rhamnose 3'-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis.,Han L, Singh S, Thorson JS, Phillips GN Jr Struct Dyn. 2016 Feb 18;3(1):012004. doi: 10.1063/1.4941368. eCollection 2016, Jan. PMID:26958582<ref>PMID:26958582</ref>
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Description: Apo structure of CalS11, TDP-rhamnose 3'-o-methyltransferase, an enzyme in Calicheamicin biosynthesis
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: G.N]]
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<div class="pdbe-citations 5hoq" style="background-color:#fffaf0;"></div>
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[[Category: Helmich, K.E]]
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== References ==
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[[Category: Han, L]]
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<references/>
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[[Category: Bingman, C.A]]
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__TOC__
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[[Category: Singh, S]]
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</StructureSection>
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[[Category: Phillips Jr]]
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[[Category: Large Structures]]
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[[Category: Thorson, J.S]]
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[[Category: Micromonospora echinospora]]
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[[Category: Enzyme Discovery For Natural Product Biosynthesis]]
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[[Category: Bingman CA]]
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[[Category: Enzyme Discovery for Natural Product Biosynthesis]]
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[[Category: Han L]]
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[[Category: Helmich KE]]
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[[Category: Phillips Jr GN]]
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[[Category: Singh S]]
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[[Category: Thorson JS]]

Current revision

Apo structure of CalS11, TDP-rhamnose 3'-o-methyltransferase, an enzyme in Calicheamicin biosynthesis

PDB ID 5hoq

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