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1c12

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[[Image:1c12.jpg|left|200px]]
 
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{{Structure
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==INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE==
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|PDB= 1c12 |SIZE=350|CAPTION= <scene name='initialview01'>1c12</scene>, resolution 2.60&Aring;
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<StructureSection load='1c12' size='340' side='right'caption='[[1c12]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=TRZ:TRAZEOLIDE'>TRZ</scene>
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<table><tr><td colspan='2'>[[1c12]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C12 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRZ:TRAZEOLIDE'>TRZ</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c12 OCA], [https://pdbe.org/1c12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c12 RCSB], [https://www.ebi.ac.uk/pdbsum/1c12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c12 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c12 OCA], [http://www.ebi.ac.uk/pdbsum/1c12 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1c12 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c1/1c12_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c12 ConSurf].
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<div style="clear:both"></div>
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'''INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE'''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Monoclonal antibodies were elicited against the small hydrophobic hapten traseolide, a commercially available musk fragrance. Antibody variable region sequences were found to belong to different sequence groups, and the binding characteristics of the corresponding antibody fragments were investigated. The antibodies M02/01/01 and M02/05/01 are highly homologous and differ in the binding pocket only at position H93. M02/05/01 (H93 Val) binds the hapten traseolide about 75-fold better than M02/01/01 (H93 Ala). A traseolide analog, missing only one methyl group, does not have the characteristic musk odorant fragrance. The antibody M02/05/01 binds this hapten analog about tenfold less tightly than the original traseolide hapten, and mimics the odorant receptor in this respect, while the antibody M02/01/01 does not distinguish between the analog and traseolide. To elucidate the structural basis for the fine specificity of binding, we determined the crystal structure of the Fab fragment of M02/05/01 complexed with the hapten at 2.6 A resolution. The crystal structure showed that only van der Waals interactions are involved in binding. The somatic Ala H93 Val mutation in M02/05/01 fills up an empty cavity in the binding pocket. This leads to an increase in binding energy and to the ability to discriminate between the hapten traseolide and its derivatives. The structural understanding of odorant specificity in an antibody gives insight in the physical principles on how specificity for such hydrophobic molecules may be achieved.
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[[Category: Large Structures]]
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==About this Structure==
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1C12 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C12 OCA].
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==Reference==
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Insight into odorant perception: the crystal structure and binding characteristics of antibody fragments directed against the musk odorant traseolide., Langedijk AC, Spinelli S, Anguille C, Hermans P, Nederlof J, Butenandt J, Honegger A, Cambillau C, Pluckthun A, J Mol Biol. 1999 Oct 1;292(4):855-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10525411 10525411]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Anguille C]]
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[[Category: Anguille, C.]]
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[[Category: Butenandt J]]
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[[Category: Butenandt, J.]]
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[[Category: Cambillau C]]
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[[Category: Cambillau, C.]]
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[[Category: Hermans P]]
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[[Category: Hermans, P.]]
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[[Category: Honegger A]]
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[[Category: Honegger, A.]]
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[[Category: Langedijk AC]]
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[[Category: Langedijk, A C.]]
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[[Category: Nederlof J]]
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[[Category: Nederlof, J.]]
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[[Category: Pluckthun A]]
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[[Category: Pluckthun, A.]]
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[[Category: Spinelli S]]
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[[Category: Spinelli, S.]]
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[[Category: antibody-antigen complex]]
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[[Category: cdrh3]]
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[[Category: immune system]]
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[[Category: musk odorant]]
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[[Category: odorant specificity]]
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[[Category: scfv fragment]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:12:23 2008''
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Current revision

INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE

PDB ID 1c12

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