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1cbf

From Proteopedia

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[[Image:1cbf.gif|left|200px]]
 
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{{Structure
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==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF==
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|PDB= 1cbf |SIZE=350|CAPTION= <scene name='initialview01'>1cbf</scene>, resolution 2.4&Aring;
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<StructureSection load='1cbf' size='340' side='right'caption='[[1cbf]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE= <scene name='pdbsite=PAL:Phosphate+Ion+Involved+In+Crystal+Contact'>PAL</scene> and <scene name='pdbsite=SAH:S-Adenosyl-Homocysteine+Binding+Site.+Crystals+Formed+In+...'>SAH</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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<table><tr><td colspan='2'>[[1cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBF FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbf OCA], [https://pdbe.org/1cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbf RCSB], [https://www.ebi.ac.uk/pdbsum/1cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbf ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbf OCA], [http://www.ebi.ac.uk/pdbsum/1cbf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cbf RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CBIF_PRIMG CBIF_PRIMG] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.<ref>PMID:23922391</ref>
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== Evolutionary Conservation ==
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'''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbf_consurf.spt"</scriptWhenChecked>
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Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1CBF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBF OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbf ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9665173 9665173]
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<references/>
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[[Category: Bacillus megaterium]]
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__TOC__
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[[Category: Precorrin-4 C(11)-methyltransferase]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Raux, E.]]
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[[Category: Priestia megaterium]]
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[[Category: Schubert, H L.]]
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[[Category: Raux E]]
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[[Category: Warren, M J.]]
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[[Category: Schubert HL]]
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[[Category: Wilson, K S.]]
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[[Category: Warren MJ]]
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[[Category: Woodcock, S C.]]
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[[Category: Wilson KS]]
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[[Category: cobalamin biosynthesis]]
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[[Category: Woodcock SC]]
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[[Category: methylase]]
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[[Category: precorrin-4 methyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:18:21 2008''
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Current revision

THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF

PDB ID 1cbf

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