2qfc
From Proteopedia
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==Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR== | ==Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR== | ||
| - | <StructureSection load='2qfc' size='340' side='right' caption='[[2qfc]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='2qfc' size='340' side='right'caption='[[2qfc]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2qfc]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2qfc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis_ATCC_35646 Bacillus thuringiensis serovar israelensis ATCC 35646]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QFC FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qfc OCA], [https://pdbe.org/2qfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qfc RCSB], [https://www.ebi.ac.uk/pdbsum/2qfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qfc ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q3EZ40_BACTI Q3EZ40_BACTI] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qf/2qfc_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qf/2qfc_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 17: | Line 20: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qfc ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qfc ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Gram-positive bacteria use a wealth of extracellular signaling peptides, so-called autoinducers, to regulate gene expression according to population densities. These "quorum sensing" systems control vital processes such as virulence, sporulation, and gene transfer. Using x-ray analysis, we determined the structure of PlcR, the major virulence regulator of the Bacillus cereus group, and obtained mechanistic insights into the effects of autoinducer binding. Our structural and phylogenetic analysis further suggests that all of those quorum sensors that bind directly to their autoinducer peptide derive from a common ancestor and form a single family (the RNPP family, for Rap/NprR/PlcR/PrgX) with conserved features. As a consequence, fundamentally different processes in different bacterial genera appear regulated by essentially the same autoinducer recognition mechanism. Our results shed light on virulence control by PlcR and elucidate origin and evolution of multicellular behavior in bacteria. | ||
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| - | Structure of PlcR: Insights into virulence regulation and evolution of quorum sensing in Gram-positive bacteria.,Declerck N, Bouillaut L, Chaix D, Rugani N, Slamti L, Hoh F, Lereclus D, Arold ST Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18490-5. Epub 2007 Nov 12. PMID:17998541<ref>PMID:17998541</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] | |
| - | + | ||
| - | == | + | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Bacillus thuringiensis serovar israelensis | + | [[Category: Bacillus thuringiensis serovar israelensis ATCC 35646]] |
| - | [[Category: Arold | + | [[Category: Large Structures]] |
| - | [[Category: Chaix | + | [[Category: Arold ST]] |
| - | [[Category: Declerck | + | [[Category: Chaix D]] |
| - | [[Category: Hoh | + | [[Category: Declerck N]] |
| - | [[Category: Rugani | + | [[Category: Hoh F]] |
| - | + | [[Category: Rugani N]] | |
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| - | + | ||
Current revision
Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR
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