1cq4

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[[Image:1cq4.gif|left|200px]]
 
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{{Structure
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==CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59==
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|PDB= 1cq4 |SIZE=350|CAPTION= <scene name='initialview01'>1cq4</scene>, resolution 1.80&Aring;
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<StructureSection load='1cq4' size='340' side='right'caption='[[1cq4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1cq4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQ4 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cq4 OCA], [https://pdbe.org/1cq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cq4 RCSB], [https://www.ebi.ac.uk/pdbsum/1cq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cq4 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cq4 OCA], [http://www.ebi.ac.uk/pdbsum/1cq4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cq4 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ICI2_HORVU ICI2_HORVU] Inhibits both subtilisin and chymotrypsin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cq4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cq4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have constructed mutants of chymotrypsin inhibitor 2 with short glutamine repeats inserted into its inhibitory loop. These mutants oligomerize when expressed in Escherichia coli. The dimer of a mutant with four glutamines now has been crystallized, and its structure has been solved by molecular replacement by using the wild-type monomer as a search model. The structure of each half of the dimer is found to be the same as that of the wild-type monomer, except around the glutamine insertion. It was proposed that the components of the oligomers are held together by hydrogen bonds between the main-chain and side-chain amides of the glutamine repeats. Instead, they appear to form by swapping domains on folding in E. coli, and the glutamine repeats connecting the components of the dimers are disordered.
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'''CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59'''
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Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat.,Chen YW, Stott K, Perutz MF Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:9990011<ref>PMID:9990011</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1cq4" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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We have constructed mutants of chymotrypsin inhibitor 2 with short glutamine repeats inserted into its inhibitory loop. These mutants oligomerize when expressed in Escherichia coli. The dimer of a mutant with four glutamines now has been crystallized, and its structure has been solved by molecular replacement by using the wild-type monomer as a search model. The structure of each half of the dimer is found to be the same as that of the wild-type monomer, except around the glutamine insertion. It was proposed that the components of the oligomers are held together by hydrogen bonds between the main-chain and side-chain amides of the glutamine repeats. Instead, they appear to form by swapping domains on folding in E. coli, and the glutamine repeats connecting the components of the dimers are disordered.
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*[[Chymotrypsin inhibitor 3D structures|Chymotrypsin inhibitor 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1CQ4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat., Chen YW, Stott K, Perutz MF, Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9990011 9990011]
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[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Chen, Y W.]]
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[[Category: Chen YW]]
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[[Category: Stott, K R.]]
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[[Category: Stott KR]]
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[[Category: immune system]]
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[[Category: polyglutamine insertion mutant]]
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[[Category: serine protease inhibitor]]
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[[Category: subtilisin- chymotrypsin inhibitor-2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:26:23 2008''
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Current revision

CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59

PDB ID 1cq4

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