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2jh8
From Proteopedia
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| - | == | + | |
| - | <StructureSection load='2jh8' size='340' side='right' caption='[[2jh8]], [[Resolution|resolution]] 3.22Å' scene=''> | + | ==The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line== |
| + | <StructureSection load='2jh8' size='340' side='right'caption='[[2jh8]], [[Resolution|resolution]] 3.22Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2jh8]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2jh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_(serotype_10_/_American_isolate) Bluetongue virus (serotype 10 / American isolate)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JH8 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.22Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GUN:GUANINE'>GUN</scene>, <scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jh8 OCA], [https://pdbe.org/2jh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jh8 RCSB], [https://www.ebi.ac.uk/pdbsum/2jh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jh8 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/Q65751_BTV1X Q65751_BTV1X] The VP4 protein is one of the five proteins (with VP1, VP3, VP6 and VP7) which form the inner capsid of the virus.[ARBA:ARBA00002541] |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/2jh8_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/2jh8_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jh8 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jh8 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Eukaryotic organisms cap the 5' ends of their messenger RNAs by a series of four chemical reactions. Some viruses achieve this using a single molecule; the crystal structure of such an enzyme from bluetongue virus reveals an elongated modular architecture that provides a scaffold for an assemblage of active sites, two contributed by a domain of novel structure. | ||
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| - | Bluetongue virus VP4 is an RNA-capping assembly line.,Sutton G, Grimes JM, Stuart DI, Roy P Nat Struct Mol Biol. 2007 May;14(5):449-51. Epub 2007 Apr 8. PMID:17417654<ref>PMID:17417654</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | |
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| - | == | + | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Grimes JM]] |
| - | [[Category: | + | [[Category: Roy P]] |
| - | [[Category: | + | [[Category: Stuart DI]] |
| - | [[Category: | + | [[Category: Sutton G]] |
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Current revision
The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line
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