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| | ==Solution NMR structure of the protease-resistent domain of Xenopus laevis ePABP2== | | ==Solution NMR structure of the protease-resistent domain of Xenopus laevis ePABP2== |
| - | <StructureSection load='2jwn' size='340' side='right' caption='[[2jwn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jwn' size='340' side='right'caption='[[2jwn]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JWN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jwn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JWN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epabp2-B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jwn OCA], [http://pdbe.org/2jwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jwn RCSB], [http://www.ebi.ac.uk/pdbsum/2jwn PDBsum], [http://www.topsan.org/Proteins/CESG/2jwn TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jwn OCA], [https://pdbe.org/2jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jwn RCSB], [https://www.ebi.ac.uk/pdbsum/2jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jwn ProSAT], [https://www.topsan.org/Proteins/CESG/2jwn TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EPA2B_XENLA EPA2B_XENLA]] Binds the poly(A) tail of mRNA. Unable to interact with the cap-binding complex and is therefore unlikely to be involved in translation initiation.<ref>PMID:15083517</ref> [UniProtKB:Q804A5] | + | [https://www.uniprot.org/uniprot/EPA2B_XENLA EPA2B_XENLA] Binds the poly(A) tail of mRNA. Unable to interact with the cap-binding complex and is therefore unlikely to be involved in translation initiation.<ref>PMID:15083517</ref> [UniProtKB:Q804A5] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/2jwn_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/2jwn_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: African clawed frog]] | + | [[Category: Large Structures]] |
| - | [[Category: Structural genomic]] | + | [[Category: Xenopus laevis]] |
| - | [[Category: Markley, J L]] | + | [[Category: Markley JL]] |
| - | [[Category: Song, J]] | + | [[Category: Song J]] |
| - | [[Category: Cesg]]
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| - | [[Category: Cytoplasm rna-binding]]
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| - | [[Category: Epabp2]]
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| - | [[Category: PSI, Protein structure initiative]]
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| - | [[Category: Rna binding protein]]
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| Structural highlights
Function
EPA2B_XENLA Binds the poly(A) tail of mRNA. Unable to interact with the cap-binding complex and is therefore unlikely to be involved in translation initiation.[1] [UniProtKB:Q804A5]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We identified a functional domain (XlePABP2-TRP) of Xenopus laevis embryonic type II poly(A)-binding protein (XlePABP2). The NMR structure of XlePABP2-TRP revealed that the protein is a homodimer formed by the antiparallel association of beta-strands from the single RNA recognition motif (RRM) domain of each subunit. In each subunit of the homodimer, the canonical RNA recognition site is occluded by a polyproline motif. Upon poly(A) binding, XlePABP2-TRP undergoes a dimer-monomer transition that removes the polyproline motif from the RNA recognition site and allows it to be replaced by the adenosine nucleotides of poly(A). Our results provide high-resolution structural information concerning type II PABPs and an example of a single RRM domain protein that transitions from a homodimer to a monomer upon RNA binding. These findings advance our understanding of RRM domain regulation, poly(A) recognition, and are relevant to understanding how type II PABPs function in mRNA processing and human disease.
Structural basis for RNA recognition by a type II poly(A)-binding protein.,Song J, McGivern JV, Nichols KW, Markley JL, Sheets MD Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15317-22. Epub 2008 Sep 29. PMID:18824697[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Good PJ, Abler L, Herring D, Sheets MD. Xenopus embryonic poly(A) binding protein 2 (ePABP2) defines a new family of cytoplasmic poly(A) binding proteins expressed during the early stages of vertebrate development. Genesis. 2004 Apr;38(4):166-75. PMID:15083517 doi:http://dx.doi.org/10.1002/gene.20015
- ↑ Song J, McGivern JV, Nichols KW, Markley JL, Sheets MD. Structural basis for RNA recognition by a type II poly(A)-binding protein. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15317-22. Epub 2008 Sep 29. PMID:18824697
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