This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3e0p
From Proteopedia
(Difference between revisions)
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
| + | |||
==The X-ray structure of Human Prostasin in complex with a covalent benzoxazole inhibitor== | ==The X-ray structure of Human Prostasin in complex with a covalent benzoxazole inhibitor== | ||
| - | <StructureSection load='3e0p' size='340' side='right' caption='[[3e0p]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='3e0p' size='340' side='right'caption='[[3e0p]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3e0p]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3e0p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E0P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B3C:BENZYL+[(1R)-1-({(2S,4R)-2-({(1S)-5-AMINO-1-[(S)-1,3-BENZOXAZOL-2-YL(HYDROXY)METHYL]PENTYL}CARBAMOYL)-4-[(4-METHYLBENZYL)OXY]PYRROLIDIN-1-YL}CARBONYL)-3-PHENYLPROPYL]CARBAMATE'>B3C</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3C:BENZYL+[(1R)-1-({(2S,4R)-2-({(1S)-5-AMINO-1-[(S)-1,3-BENZOXAZOL-2-YL(HYDROXY)METHYL]PENTYL}CARBAMOYL)-4-[(4-METHYLBENZYL)OXY]PYRROLIDIN-1-YL}CARBONYL)-3-PHENYLPROPYL]CARBAMATE'>B3C</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3e0n|3e0n]], [[3e16|3e16]], [[3e1x|3e1x]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3e0n|3e0n]], [[3e16|3e16]], [[3e1x|3e1x]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRSS8 ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRSS8 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e0p OCA], [https://pdbe.org/3e0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e0p RCSB], [https://www.ebi.ac.uk/pdbsum/3e0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e0p ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/PRSS8_HUMAN PRSS8_HUMAN]] Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).<ref>PMID:15246975</ref> <ref>PMID:15474520</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/3e0p_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/3e0p_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 34: | Line 35: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Harris, J L]] | [[Category: Harris, J L]] | ||
[[Category: Hornsby, M]] | [[Category: Hornsby, M]] | ||
Current revision
The X-ray structure of Human Prostasin in complex with a covalent benzoxazole inhibitor
| |||||||||||
Categories: Human | Large Structures | Harris, J L | Hornsby, M | Lesley, S A | Shipway, A | Spraggon, G | Benzoxazole | Cell membrane | Channel | Enac | Glycoprotein | Hydrolase | Membrane | Prostasin | Protease | Secreted | Serine protease | Transmembrane | Warhead | Zymogen
