2zwk

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==Crystal structure of intimin-Tir90 complex==
==Crystal structure of intimin-Tir90 complex==
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<StructureSection load='2zwk' size='340' side='right' caption='[[2zwk]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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<StructureSection load='2zwk' size='340' side='right'caption='[[2zwk]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2zwk]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_0157:h7_edl933 Escherichia coli 0157:h7 edl933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZWK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2zwk]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._EDL933 Escherichia coli O157:H7 str. EDL933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZWK FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zqk|2zqk]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">intimin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155864 Escherichia coli 0157:H7 EDL933]), tir ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155864 Escherichia coli 0157:H7 EDL933])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zwk OCA], [https://pdbe.org/2zwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zwk RCSB], [https://www.ebi.ac.uk/pdbsum/2zwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zwk ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zwk OCA], [http://pdbe.org/2zwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zwk RCSB], [http://www.ebi.ac.uk/pdbsum/2zwk PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57]] Necessary for the production of attaching and effacing lesions on tissue culture cells. [[http://www.uniprot.org/uniprot/TIR_ECO57 TIR_ECO57]] Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion. In strain EDL933, acts via the effector protein EspF(U), in a phosphotyrosine- and NCK-independent manner. Tir binds to host BAIAP2, which mediates association with EspF(U) and leads to stimulation of actin polymerization.<ref>PMID:10225900</ref> <ref>PMID:14764108</ref> <ref>PMID:16922867</ref> <ref>PMID:17521329</ref>
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[https://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57] Necessary for the production of attaching and effacing lesions on tissue culture cells.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/2zwk_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/2zwk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zwk ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zwk ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Escherichia coli 0157:h7 edl933]]
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[[Category: Escherichia coli O157:H7 str. EDL933]]
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[[Category: Gao, F]]
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[[Category: Large Structures]]
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[[Category: Gao, G F]]
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[[Category: Gao F]]
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[[Category: Li, D F]]
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[[Category: Gao GF]]
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[[Category: Ma, Y]]
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[[Category: Li D-F]]
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[[Category: Cell adhesion]]
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[[Category: Ma Y]]
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[[Category: Cell membrane]]
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[[Category: Cell outer membrane]]
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[[Category: Membrane]]
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[[Category: Protein-protein complex]]
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[[Category: Receptor]]
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[[Category: Transmembrane]]
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[[Category: Unique intimin-tir octamer intermediate]]
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[[Category: Virulence]]
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Current revision

Crystal structure of intimin-Tir90 complex

PDB ID 2zwk

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