|
|
| (11 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1d65.gif|left|200px]] | |
| | | | |
| - | {{Structure
| + | ==MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER TWIST AND MINOR-GROOVE WATER STRUCTURE AT 2.2 ANGSTROMS RESOLUTION== |
| - | |PDB= 1d65 |SIZE=350|CAPTION= <scene name='initialview01'>1d65</scene>, resolution 2.200Å
| + | <StructureSection load='1d65' size='340' side='right'caption='[[1d65]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1d65]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D65 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d65 OCA], [https://pdbe.org/1d65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d65 RCSB], [https://www.ebi.ac.uk/pdbsum/1d65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d65 ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d65 OCA], [http://www.ebi.ac.uk/pdbsum/1d65 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d65 RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Brown DG]] |
| - | '''MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER TWIST AND MINOR-GROOVE WATER STRUCTURE AT 2.2 ANGSTROMS RESOLUTION'''
| + | [[Category: Edwards KJ]] |
| - | | + | [[Category: Neidle S]] |
| - | | + | [[Category: Spink N]] |
| - | ==Overview==
| + | |
| - | The crystal structure of the dodecanucleotide duplex d(CGCAAATTTGCG)2 has been solved to 2.2 A resolution and refined to an R-factor of 18.1% with the inclusion of 71 water molecules. The structure shows propeller twists of up to -20 degrees for the A.T base-pairs, although there is probably only one (weak) three-centre hydrogen bond in the six base-pair AT narrow minor-groove region. An extensive ribbon of hydration has been located in this groove that has features distinctive from the classic "spine of hydration". Solvation around phosphate groups is described, with several instances of water molecules bridging between phosphates.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1D65 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D65 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG)2. An examination of propeller twist and minor-groove water structure at 2.2 A resolution., Edwards KJ, Brown DG, Spink N, Skelly JV, Neidle S, J Mol Biol. 1992 Aug 20;226(4):1161-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1518049 1518049]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Brown, D G.]] | + | |
| - | [[Category: Edwards, K J.]] | + | |
| - | [[Category: Neidle, S.]] | + | |
| - | [[Category: Spink, N.]] | + | |
| - | [[Category: b-dna]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:35:23 2008''
| + | |
