1d6s

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[[Image:1d6s.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE==
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|PDB= 1d6s |SIZE=350|CAPTION= <scene name='initialview01'>1d6s</scene>, resolution 2.30&Aring;
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<StructureSection load='1d6s' size='340' side='right'caption='[[1d6s]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
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<table><tr><td colspan='2'>[[1d6s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D6S FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d6s OCA], [https://pdbe.org/1d6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d6s RCSB], [https://www.ebi.ac.uk/pdbsum/1d6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d6s ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1oas|1OAS]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d6s OCA], [http://www.ebi.ac.uk/pdbsum/1d6s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d6s RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CYSK_SALTY CYSK_SALTY] Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.
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== Evolutionary Conservation ==
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'''CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d6/1d6s_consurf.spt"</scriptWhenChecked>
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Covalent binding of L-methionine as an external aldimine to the pyridoxal 5'-phosphate-cofactor in the K41A mutant of O-acetylserine sulfhydrylase from Salmonella typhimurium induces a large conformational change in the protein. Methionine mimics the action of the substrate O-acetyl-L-serine during catalysis. The alpha-carboxylate moiety of L-methionine in external aldimine linkage with the active site pyridoxal 5'-phosphate forms a hydrogen bonding network to the "asparagine-loop" P67-T68-N69-G70 which adopts a different conformation than in the native protein. The side-chain nitrogen of Asn69 moves more than 7 A to make a hydrogen bond to the alpha-carboxylate group of the inhibitor. As the external aldimine is formed, the PLP tilts by 13 degrees along its longitudinal axis such that C4' moves toward the entrance to the active site and the side-chain of the methionine is directed toward the active site entrance. The local rearrangement acts as a trigger to induce a large global conformational change in the protein. A subdomain comprised of beta-strand 4, alpha-helix 3, beta-strand 5 and alpha-helix 4 moves towards the active site by a rotation of 7 degrees. This subdomain movement results in a reduction of the severe twist of its central beta-sheet and reduces the active site entrance to a small hole, giving access only to small molecules like sulfide, the second substrate, or acetate, the first product.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1D6S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6S OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d6s ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Ligand binding induces a large conformational change in O-acetylserine sulfhydrylase from Salmonella typhimurium., Burkhard P, Tai CH, Ristroph CM, Cook PF, Jansonius JN, J Mol Biol. 1999 Aug 27;291(4):941-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10452898 10452898]
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</StructureSection>
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[[Category: Cysteine synthase]]
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[[Category: Large Structures]]
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[[Category: Salmonella typhimurium]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Single protein]]
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[[Category: Burkhard P]]
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[[Category: Burkhard, P.]]
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[[Category: Cook PF]]
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[[Category: Cook, P F.]]
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[[Category: Jansonius JN]]
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[[Category: Jansonius, J N.]]
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[[Category: Ristroph CM]]
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[[Category: Ristroph, C M.]]
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[[Category: Tai CH]]
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[[Category: Tai, C H.]]
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[[Category: beta replacement enzyme]]
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[[Category: cysteine biosynthesis]]
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[[Category: k41a]]
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[[Category: plp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:35:47 2008''
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Current revision

CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE

PDB ID 1d6s

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