1d7q

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[[Image:1d7q.jpg|left|200px]]
 
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{{Structure
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==HUMAN TRANSLATION INITIATION FACTOR EIF1A==
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|PDB= 1d7q |SIZE=350|CAPTION= <scene name='initialview01'>1d7q</scene>
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<StructureSection load='1d7q' size='340' side='right'caption='[[1d7q]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1d7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D7Q FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7q OCA], [https://pdbe.org/1d7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d7q RCSB], [https://www.ebi.ac.uk/pdbsum/1d7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d7q ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7q OCA], [http://www.ebi.ac.uk/pdbsum/1d7q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d7q RCSB]</span>
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[https://www.uniprot.org/uniprot/IF1AX_HUMAN IF1AX_HUMAN] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/1d7q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d7q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.
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'''HUMAN TRANSLATION INITIATION FACTOR EIF1A'''
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The eIF1A solution structure reveals a large RNA-binding surface important for scanning function.,Battiste JL, Pestova TV, Hellen CU, Wagner G Mol Cell. 2000 Jan;5(1):109-19. PMID:10678173<ref>PMID:10678173</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1d7q" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1D7Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7Q OCA].
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__TOC__
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</StructureSection>
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==Reference==
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The eIF1A solution structure reveals a large RNA-binding surface important for scanning function., Battiste JL, Pestova TV, Hellen CU, Wagner G, Mol Cell. 2000 Jan;5(1):109-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10678173 10678173]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Battiste, J L.]]
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[[Category: Battiste JL]]
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[[Category: Hellen, C U.T.]]
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[[Category: Hellen CUT]]
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[[Category: Pestova, T V.]]
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[[Category: Pestova TV]]
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[[Category: Wagner, G.]]
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[[Category: Wagner G]]
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[[Category: beta-barrel]]
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[[Category: ob-fold]]
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[[Category: rna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:36:20 2008''
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Current revision

HUMAN TRANSLATION INITIATION FACTOR EIF1A

PDB ID 1d7q

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