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| - | [[Image:1d90.gif|left|200px]] | |
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| - | {{Structure
| + | ==REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS== |
| - | |PDB= 1d90 |SIZE=350|CAPTION= <scene name='initialview01'>1d90</scene>, resolution 1.700Å
| + | <StructureSection load='1d90' size='340' side='right'caption='[[1d90]], [[Resolution|resolution]] 1.70Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DI:2'-DEOXYINOSINE-5'-MONOPHOSPHATE'>DI</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1d90]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D90 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d90 OCA], [https://pdbe.org/1d90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d90 RCSB], [https://www.ebi.ac.uk/pdbsum/1d90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d90 ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d90 OCA], [http://www.ebi.ac.uk/pdbsum/1d90 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d90 RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Aymani J]] |
| - | '''REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS'''
| + | [[Category: Brown T]] |
| - | | + | [[Category: Cruse WBT]] |
| - | | + | [[Category: Jack AGC]] |
| - | ==Overview==
| + | [[Category: Kennard O]] |
| - | The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7A. The sequence crystallises in space group P6(1), with unit cell dimensions a = b = 45.07, c = 45.49A. The refinement converged with a crystallographic residual R = 0.14 and the location of 81 solvent molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C) base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure shows it to be essentially isomorphous with that reported for d(GGGGCTCC) with the mispairs adopting a "wobble" conformation. Conformational parameters and base stacking interactions are compared to those for the native duplex d(GGGGCCCC) and other similar sequences. A rationale for the apparent increased crystal packing efficiency and lattice stability of the I.T octamer is given.
| + | [[Category: Leonard GA]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1D90 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs., Cruse WB, Aymani J, Kennard O, Brown T, Jack AG, Leonard GA, Nucleic Acids Res. 1989 Jan 11;17(1):55-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2911488 2911488]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Aymani, J.]] | + | |
| - | [[Category: Brown, T.]] | + | |
| - | [[Category: Cruse, W B.T.]] | + | |
| - | [[Category: Jack, A G.C.]] | + | |
| - | [[Category: Kennard, O.]] | + | |
| - | [[Category: Leonard, G A.]] | + | |
| - | [[Category: a-dna]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: mismatched]]
| + | |
| - | [[Category: modified]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:36:57 2008''
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