3coo

From Proteopedia

(Difference between revisions)

Current revision

The crystal structure of Reelin-N domain of F-spondin

Structural highlights

3coo is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPON1_HUMAN Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The extracellular matrix protein F-spondin mediates axon guidance during neuronal development. Its N-terminal domain, termed the reelin-N domain, is conserved in F-spondins, reelins, and other extracellular matrix proteins. In this study, a recombinant human reelin-N domain has been expressed, purified, and shown to bind heparin. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. Substantial conformational variations even in secondary structure are observed between the two chemically identical reelin-N domains in one crystallographic asymmetric unit. The variations may result from extensive, highly specific interactions across the interface of the two reelin-N domains. The calculated values of buried surface area and the interface's shape complementarity are consistent with the formation of a weak dimer. The homophilic asymmetric dimer can potentially offer advantages in binding to ligands such as glycosaminoglycans, which may, in turn, bridge the two reelin-N domains and stabilize the dimer.

The crystal structure of the heparin-binding reelin-N domain of f-spondin.,Tan K, Duquette M, Liu JH, Lawler J, Wang JH J Mol Biol. 2008 Sep 19;381(5):1213-23. Epub 2008 Jun 24. PMID:18602404^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tan K, Duquette M, Liu JH, Lawler J, Wang JH. The crystal structure of the heparin-binding reelin-N domain of f-spondin. J Mol Biol. 2008 Sep 19;381(5):1213-23. Epub 2008 Jun 24. PMID:18602404 doi:10.1016/j.jmb.2008.06.045

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3coo"

Categories: Homo sapiens | Large Structures | Lawler J | Tan K | Wang JH

@@ Line 1: / Line 1: @@
 ==The crystal structure of Reelin-N domain of F-spondin==
-<StructureSection load='3coo' size='340' side='right' caption='[[3coo]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='3coo' size='340' side='right'caption='[[3coo]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3coo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3COO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3COO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3coo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3COO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3COO FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Homo sapiens ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3coo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3coo OCA], [http://pdbe.org/3coo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3coo RCSB], [http://www.ebi.ac.uk/pdbsum/3coo PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3coo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3coo OCA], [https://pdbe.org/3coo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3coo RCSB], [https://www.ebi.ac.uk/pdbsum/3coo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3coo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SPON1_HUMAN SPON1_HUMAN]] Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
+[https://www.uniprot.org/uniprot/SPON1_HUMAN SPON1_HUMAN] Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/co/3coo_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/co/3coo_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 33: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Lawler, J]]
+[[Category: Large Structures]]
-[[Category: Tan, K]]
+[[Category: Lawler J]]
-[[Category: Wang, J H]]
+[[Category: Tan K]]
-[[Category: Cell adhesion]]
+[[Category: Wang JH]]
-[[Category: Extracellular matrix]]
-[[Category: F-spondin]]
-[[Category: Glycoprotein]]
-[[Category: Reelin-n domain]]
-[[Category: Secreted]]

3coo

From Proteopedia

Current revision

The crystal structure of Reelin-N domain of F-spondin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox