1d9v

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[[Image:1d9v.jpg|left|200px]]
 
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{{Structure
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==HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM==
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|PDB= 1d9v |SIZE=350|CAPTION= <scene name='initialview01'>1d9v</scene>, resolution 1.75&Aring;
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<StructureSection load='1d9v' size='340' side='right'caption='[[1d9v]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1d9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D9V FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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|GENE= HITA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d9v OCA], [https://pdbe.org/1d9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d9v RCSB], [https://www.ebi.ac.uk/pdbsum/1d9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d9v ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1mrp|1MRP]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d9v OCA], [http://www.ebi.ac.uk/pdbsum/1d9v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d9v RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/FBPA_HAEIN FBPA_HAEIN] Part of the ABC transporter complex FbpABC (TC 3.A.1.10.1) involved in Fe(3+) ions import. This protein specifically binds Fe(3+) and is involved in its transmembrane transport (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d9/1d9v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d9v ConSurf].
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<div style="clear:both"></div>
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'''HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM'''
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==See Also==
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*[[Ferric-binding protein|Ferric-binding protein]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of the iron-free (apo) form of the Haemophilus influenzae Fe(3+)-binding protein (hFbp) has been determined to 1.75 A resolution. Information from this structure complements that derived from the holo structure with respect to the delineation of the process of iron binding and release. A 21 degrees rotation separates the two structural domains when the apo form is compared with the holo conformer, indicating that upon release of iron, the protein undergoes a change in conformation by bending about the central beta-sheet hinge. A surprising finding in the apo-hFbp structure was that the ternary binding site anion, observed in the crystals as phosphate, remained bound. In solution, apo-hFbp bound phosphate with an affinity K(d) of 2.3 x 10(-3) M. The presence of this ternary binding site anion appears to arrange the C-terminal iron-binding residues conducive to complementary binding to Fe(3+), while residues in the N-terminal binding domain must undergo induced fit to accommodate the Fe(3+) ligand. These observations suggest a binding process, the first step of which is the binding of a synergistic anion such as phosphate to the C-terminal domain. Next, iron binds to the preordered half-site on the C-terminal domain. Finally, the presence of iron organizes the N-terminal half-site and closes the interdomain hinge. The use of the synergistic anion and this iron binding process results in an extremely high affinity of the Fe(3+)-binding proteins for Fe(3+) (nFbp K'(eff) = 2.4 x 10(18) M(-1)). This high-affinity ligand binding process is unique among the family of bacterial periplasmic binding proteins and has interesting implications in the mechanism of iron removal from the Fe(3+)-binding proteins during FbpABC-mediated iron transport across the cytoplasmic membrane.
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==About this Structure==
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1D9V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9V OCA].
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==Reference==
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Crystallographic and biochemical analyses of the metal-free Haemophilus influenzae Fe3+-binding protein., Bruns CM, Anderson DS, Vaughan KG, Williams PA, Nowalk AJ, McRee DE, Mietzner TA, Biochemistry. 2001 Dec 25;40(51):15631-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11747438 11747438]
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[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bruns, C M.]]
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[[Category: Bruns CM]]
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[[Category: McRee, D E.]]
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[[Category: McRee DE]]
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[[Category: Williams, P A.]]
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[[Category: Williams PA]]
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[[Category: abc cassette receptor protein]]
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[[Category: apo form]]
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[[Category: binding protein]]
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[[Category: ferric]]
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[[Category: iron]]
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[[Category: periplasmic protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:37:26 2008''
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Current revision

HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM

PDB ID 1d9v

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