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2phn
From Proteopedia
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==Crystal structure of an amide bond forming F420-gamma glutamyl ligase from Archaeoglobus fulgidus== | ==Crystal structure of an amide bond forming F420-gamma glutamyl ligase from Archaeoglobus fulgidus== | ||
| - | <StructureSection load='2phn' size='340' side='right' caption='[[2phn]], [[Resolution|resolution]] 1.35Å' scene=''> | + | <StructureSection load='2phn' size='340' side='right'caption='[[2phn]], [[Resolution|resolution]] 1.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2phn]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2phn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PHN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2phn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2phn OCA], [https://pdbe.org/2phn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2phn RCSB], [https://www.ebi.ac.uk/pdbsum/2phn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2phn ProSAT], [https://www.topsan.org/Proteins/MCSG/2phn TOPSAN]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/COFE_ARCFU COFE_ARCFU] Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2), with a gamma-linkage between the two glutamates. May be able to add up to four gamma-linked glutamates, since F420-4 is a species that was isolated from A.fulgidus.<ref>PMID:17669425</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ph/2phn_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ph/2phn_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 2phn" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2phn" style="background-color:#fffaf0;"></div> | ||
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| - | ==See Also== | ||
| - | *[[Gamma-Glutamyl Ligase|Gamma-Glutamyl Ligase]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Archaeoglobus fulgidus DSM 4304]] |
| - | [[Category: Edwards | + | [[Category: Large Structures]] |
| - | [[Category: Evdokimova | + | [[Category: Edwards A]] |
| - | [[Category: Joachimiak | + | [[Category: Evdokimova E]] |
| - | [[Category: Kudritska | + | [[Category: Joachimiak A]] |
| - | + | [[Category: Kudritska M]] | |
| - | [[Category: Nocek | + | [[Category: Nocek B]] |
| - | [[Category: Savchenko | + | [[Category: Savchenko A]] |
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Current revision
Crystal structure of an amide bond forming F420-gamma glutamyl ligase from Archaeoglobus fulgidus
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