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| - | [[Image:1ddp.gif|left|200px]] | |
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| - | {{Structure
| + | ==Solution structure of a CISPLATIN-INDUCED [CATAGCTATG]2 Interstrand cross-link== |
| - | |PDB= 1ddp |SIZE=350|CAPTION= <scene name='initialview01'>1ddp</scene>
| + | <StructureSection load='1ddp' size='340' side='right'caption='[[1ddp]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1ddp]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DDP FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPT:CISPLATIN'>CPT</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ddp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddp OCA], [https://pdbe.org/1ddp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ddp RCSB], [https://www.ebi.ac.uk/pdbsum/1ddp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ddp ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ddp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddp OCA], [http://www.ebi.ac.uk/pdbsum/1ddp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ddp RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK'''
| + | [[Category: Drobny GP]] |
| - | | + | [[Category: Hopkins PB]] |
| - | | + | [[Category: Huang H]] |
| - | ==Overview==
| + | [[Category: Reid BR]] |
| - | The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
| + | [[Category: Zhu L]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1DDP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8525382 8525382]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Drobny, G P.]] | + | |
| - | [[Category: Hopkins, P B.]] | + | |
| - | [[Category: Huang, H.]] | + | |
| - | [[Category: Reid, B R.]] | + | |
| - | [[Category: Zhu, L.]] | + | |
| - | [[Category: antitumor drug]]
| + | |
| - | [[Category: cis-ddp]]
| + | |
| - | [[Category: dna]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: interstrand cross-link]]
| + | |
| - | [[Category: nmr]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:39:35 2008''
| + | |
