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| - | [[Image:1ddz.gif|left|200px]] | |
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| - | {{Structure
| + | ==X-RAY STRUCTURE OF A BETA-CARBONIC ANHYDRASE FROM THE RED ALGA, PORPHYRIDIUM PURPUREUM R-1== |
| - | |PDB= 1ddz |SIZE=350|CAPTION= <scene name='initialview01'>1ddz</scene>, resolution 2.2Å
| + | <StructureSection load='1ddz' size='340' side='right'caption='[[1ddz]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
| + | <table><tr><td colspan='2'>[[1ddz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyridium_purpureum Porphyridium purpureum]. The January 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Carbonic Anhydrase'' by Shuchismita Dutta and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_1 10.2210/rcsb_pdb/mom_2004_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DDZ FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | |DOMAIN= | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ddz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddz OCA], [https://pdbe.org/1ddz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ddz RCSB], [https://www.ebi.ac.uk/pdbsum/1ddz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ddz ProSAT]</span></td></tr> |
| - | |RELATEDENTRY= | + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ddz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddz OCA], [http://www.ebi.ac.uk/pdbsum/1ddz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ddz RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/Q43060_PORPP Q43060_PORPP] |
| | | | |
| - | '''X-RAY STRUCTURE OF A BETA-CARBONIC ANHYDRASE FROM THE RED ALGA, PORPHYRIDIUM PURPUREUM R-1'''
| + | ==See Also== |
| - | | + | *[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The carbonic anhydrases (CAs) fall into three evolutionarily distinct families designated alpha-, beta-, and gamma-CAs based on their primary structure. beta-CAs are present in higher plants, algae, and prokaryotes, and are involved in inorganic carbon utilization. Here, we describe the novel x-ray structure of beta-CA from the red alga, Porphyridium purpureum, at 2.2-A resolution using intrinsic zinc multiwavelength anomalous diffraction phasing. The CA monomer is composed of two internally repeating structures, being folded as a pair of fundamentally equivalent motifs of an alpha/beta domain and three projecting alpha-helices. The motif is obviously distinct from that of either alpha- or gamma-CAs. This homodimeric CA appears like a tetramer with a pseudo 222 symmetry. The active site zinc is coordinated by a Cys-Asp-His-Cys tetrad that is strictly conserved among the beta-CAs. No water molecule is found in a zinc-liganding radius, indicating that the zinc-hydroxide mechanism in alpha-CAs, and possibly in gamma-CAs, is not directly applicable to the case in beta-CAs. Zinc coordination environments of the CAs provide an interesting example of the convergent evolution of distinct catalytic sites required for the same CO(2) hydration reaction.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1DDZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Porphyridium_purpureum Porphyridium purpureum]. The following page contains interesting information on the relation of 1DDZ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb49_1.html Carbonic Anhydrase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDZ OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | X-ray structure of beta-carbonic anhydrase from the red alga, Porphyridium purpureum, reveals a novel catalytic site for CO(2) hydration., Mitsuhashi S, Mizushima T, Yamashita E, Yamamoto M, Kumasaka T, Moriyama H, Ueki T, Miyachi S, Tsukihara T, J Biol Chem. 2000 Feb 25;275(8):5521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10681531 10681531]
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| - | [[Category: Carbonate dehydratase]]
| + | |
| | [[Category: Carbonic Anhydrase]] | | [[Category: Carbonic Anhydrase]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Porphyridium purpureum]] | | [[Category: Porphyridium purpureum]] |
| - | [[Category: Single protein]] | + | [[Category: RCSB PDB Molecule of the Month]] |
| - | [[Category: Mitsuhashi, S.]] | + | [[Category: Mitsuhashi S]] |
| - | [[Category: Miyachi, S.]] | + | [[Category: Miyachi S]] |
| - | [[Category: Mizushima, T.]] | + | [[Category: Mizushima T]] |
| - | [[Category: Tsukihara, T.]] | + | [[Category: Tsukihara T]] |
| - | [[Category: Yamashita, E.]] | + | [[Category: Yamashita E]] |
| - | [[Category: alpha-beta-alpha]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:39:41 2008''
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