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2erw

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Crystal Structure of Infestin 4, a factor XIIa inhibitor

Structural highlights

2erw is a 1 chain structure with sequence from Triatoma infestans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q95P16_TRIIF

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Blood coagulation is an important process in haemostasis, and disorders of blood coagulation can lead to an increased risk of haemorrhage and thrombosis. Coagulation is highly conserved in mammals and has been comprehensively studied in humans in the investigation of bleeding or thrombotic diseases. Some substances can act as inhibitors of blood coagulation and may affect one or multiple enzymes throughout the process. A specific thrombin inhibitor called infestin has been isolated from the midgut of the haematophagous insect Triatoma infestans. Infestin is a member of the nonclassical Kazal-type serine protease inhibitors and is composed of four domains, all of which have a short central alpha-helix and a small antiparallel beta-sheet. Domains 1 and 4 of infestin (infestins 1 and 4) possess specific inhibitory activities. Infestin 1 inhibits thrombin, while infestin 4 is an inhibitor of factor XIIa, plasmin and factor Xa. Here, the structure determination and structural analysis of infestin 1 complexed with trypsin and of infestin 4 alone are reported. Through molecular modelling and docking, it is suggested that the protein-protein binding site is conserved in the infestin 1-thrombin complex compared with other Kazal-type inhibitors. Infestin 4 is able to bind factor XIIa, and the F9N and N11R mutants selected by phage display were shown to be more selective for factor XIIa in comparison to the wild type.

The Kazal-type inhibitors infestins 1 and 4 differ in specificity but are similar in three-dimensional structure.,Campos IT, Souza TA, Torquato RJ, De Marco R, Tanaka-Azevedo AM, Tanaka AS, Barbosa JA Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):695-702. Epub 2012 May 17. PMID:22683792^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Campos IT, Souza TA, Torquato RJ, De Marco R, Tanaka-Azevedo AM, Tanaka AS, Barbosa JA. The Kazal-type inhibitors infestins 1 and 4 differ in specificity but are similar in three-dimensional structure. Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):695-702. Epub 2012 May 17. PMID:22683792 doi:10.1107/S0907444912009067

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2erw"

Categories: Large Structures | Triatoma infestans | Barbosa JARG | Campos ITN | Tanaka AS

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Infestin 4, a factor XIIa inhibitor==
-<StructureSection load='2erw' size='340' side='right' caption='[[2erw]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+<StructureSection load='2erw' size='340' side='right'caption='[[2erw]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2erw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Assassin_bug Assassin bug]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ERW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2erw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triatoma_infestans Triatoma infestans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ERW FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tbr|1tbr]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2erw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2erw OCA], [http://pdbe.org/2erw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2erw RCSB], [http://www.ebi.ac.uk/pdbsum/2erw PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2erw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2erw OCA], [https://pdbe.org/2erw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2erw RCSB], [https://www.ebi.ac.uk/pdbsum/2erw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2erw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q95P16_TRIIF Q95P16_TRIIF]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2erw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2erw_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Assassin bug]]
+[[Category: Large Structures]]
-[[Category: Barbosa, J A.R G]]
+[[Category: Triatoma infestans]]
-[[Category: Campos, I T.N]]
+[[Category: Barbosa JARG]]
-[[Category: Tanaka, A S]]
+[[Category: Campos ITN]]
-[[Category: Blood clotting]]
+[[Category: Tanaka AS]]
-[[Category: Hydrolase inhibitor]]
-[[Category: Kazal type domain]]

2erw

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Current revision

Crystal Structure of Infestin 4, a factor XIIa inhibitor

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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