1mc8

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==Crystal Structure of Flap Endonuclease-1 R42E mutant from Pyrococcus horikoshii==
==Crystal Structure of Flap Endonuclease-1 R42E mutant from Pyrococcus horikoshii==
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<StructureSection load='1mc8' size='340' side='right' caption='[[1mc8]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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<StructureSection load='1mc8' size='340' side='right'caption='[[1mc8]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1mc8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MC8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1mc8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MC8 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mc8 OCA], [http://pdbe.org/1mc8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mc8 RCSB], [http://www.ebi.ac.uk/pdbsum/1mc8 PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mc8 OCA], [https://pdbe.org/1mc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mc8 RCSB], [https://www.ebi.ac.uk/pdbsum/1mc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mc8 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/FEN_PYRHO FEN_PYRHO]] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).
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[https://www.uniprot.org/uniprot/FEN_PYRHO FEN_PYRHO] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/1mc8_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/1mc8_consurf.spt"</scriptWhenChecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
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==See Also==
==See Also==
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*[[Endonuclease|Endonuclease]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pyrococcus shinkaii]]
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[[Category: Large Structures]]
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[[Category: Abe, J]]
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[[Category: Pyrococcus horikoshii]]
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[[Category: Harata, K]]
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[[Category: Abe J]]
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[[Category: Matsui, E]]
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[[Category: Harata K]]
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[[Category: Matsui, I]]
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[[Category: Matsui E]]
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[[Category: Musti, K V]]
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[[Category: Matsui I]]
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[[Category: Yamazaki, K]]
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[[Category: Musti KV]]
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[[Category: Flexible loop]]
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[[Category: Yamazaki K]]
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[[Category: Hydrolase]]
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Current revision

Crystal Structure of Flap Endonuclease-1 R42E mutant from Pyrococcus horikoshii

PDB ID 1mc8

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