1dp8

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[[Image:1dp8.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE NITRIC OXIDE BOUND FIXL HEME DOMAIN==
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|PDB= 1dp8 |SIZE=350|CAPTION= <scene name='initialview01'>1dp8</scene>, resolution 2.5&Aring;
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<StructureSection load='1dp8' size='340' side='right'caption='[[1dp8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITROGEN+OXIDE'>NO</scene>
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<table><tr><td colspan='2'>[[1dp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DP8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dp8 OCA], [https://pdbe.org/1dp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dp8 RCSB], [https://www.ebi.ac.uk/pdbsum/1dp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dp8 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1bv6|1BV6]], [[1bv5|1BV5]], [[1dp6|1DP6]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dp8 OCA], [http://www.ebi.ac.uk/pdbsum/1dp8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dp8 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/FIXL_BRADU FIXL_BRADU]
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== Evolutionary Conservation ==
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'''CRYSTAL STRUCTURE OF THE NITRIC OXIDE BOUND FIXL HEME DOMAIN'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/1dp8_consurf.spt"</scriptWhenChecked>
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The FixL heme domain serves as the dioxygen switch in the FixL/FixJ two-component system of Rhizobia. Recent structural studies of the Bradyrhizobium japonicum FixL heme domain (BjFixLH) have suggested an allosteric mechanism that is distinct from the classical hemoglobin model. To gain further insight into the FixL sensing mechanism, structures of BjFixLH bound to dioxygen, imidazole, and nitric oxide have been determined. These structures, particularly the structure of BjFixLH bound to its physiological ligand, dioxygen, have helped to address a number of important issues relevant to the BjFixLH sensing mechanism. On the basis of the oxy-BjFixLH structure, a conserved arginine is found to stabilize the dioxygen ligand in a mode reminiscent of the distal histidine in classical myoglobins and hemoglobins. The structure of BjFixLH bound to imidazole elucidates the structural requirements for accommodating sterically bulky ligands. Finally, the structure of BjFixLH bound to nitric oxide provides evidence for a structural intermediate in the heme-driven conformational change.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1DP8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DP8 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dp8 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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New mechanistic insights from structural studies of the oxygen-sensing domain of Bradyrhizobium japonicum FixL., Gong W, Hao B, Chan MK, Biochemistry. 2000 Apr 11;39(14):3955-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10747783 10747783]
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</StructureSection>
[[Category: Bradyrhizobium japonicum]]
[[Category: Bradyrhizobium japonicum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chan, M K.]]
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[[Category: Chan MK]]
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[[Category: Gong, W.]]
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[[Category: Gong W]]
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[[Category: Hao, B.]]
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[[Category: Hao B]]
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[[Category: fixl heme domain liganded structure]]
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[[Category: heme]]
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[[Category: histidine kinase]]
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[[Category: signal transduction]]
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[[Category: two component system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:45:57 2008''
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Current revision

CRYSTAL STRUCTURE OF THE NITRIC OXIDE BOUND FIXL HEME DOMAIN

PDB ID 1dp8

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