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| - | [[Image:1dpg.gif|left|200px]] | |
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| - | {{Structure
| + | ==GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES== |
| - | |PDB= 1dpg |SIZE=350|CAPTION= <scene name='initialview01'>1dpg</scene>, resolution 2.0Å
| + | <StructureSection load='1dpg' size='340' side='right'caption='[[1dpg]], [[Resolution|resolution]] 2.00Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
| + | <table><tr><td colspan='2'>[[1dpg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuconostoc_mesenteroides Leuconostoc mesenteroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DPG FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_1-dehydrogenase Glucose-6-phosphate 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.49 1.1.1.49] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | |GENE= G6PD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1245 Leuconostoc mesenteroides])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dpg OCA], [https://pdbe.org/1dpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dpg RCSB], [https://www.ebi.ac.uk/pdbsum/1dpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dpg ProSAT]</span></td></tr> |
| - | |RELATEDENTRY= | + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dpg OCA], [http://www.ebi.ac.uk/pdbsum/1dpg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dpg RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/G6PD_LEUME G6PD_LEUME] |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/1dpg_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dpg ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES'''
| + | ==See Also== |
| - | | + | *[[Glucose 6-phosphate dehydrogenase|Glucose 6-phosphate dehydrogenase]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | BACKGROUND: Glucose 6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate pathway. Normally the pathway is synthetic and NADP-dependent, but the Gram-positive bacterium Leuconostoc mesenteroides, which does not have a complete glycolytic pathway, also uses the oxidative enzymes of the pentose phosphate pathway for catabolic reactions, and selects either NAD or NADP depending on the demands for catabolic or anabolic metabolism. RESULTS: The structure of G6PD has been determined and refined to 2.0 A resolution. The enzyme is a dimer, each subunit consisting of two domains. The smaller domain is a classic dinucleotide-binding fold, while the larger one is a new beta+ alpha fold, not previously seen, with a predominantly antiparallel nine-stranded beta-sheet. There are significant structural differences in the coenzyme-binding domains of the two subunits, caused by Pro 149 which is cis in one subunit and trans in the other. CONCLUSIONS: The structure has allowed us to propose the location of the active site and the coenzyme-binding site, and suggests the role of many of the residues conserved between species. We propose that the conserved Arg46 would interact with both the adenine ring and the 2'-phosphate of NADP. Gln47, which is not conserved, may contribute to the change from NADP to dual coenzyme specificity. His178, in a nine-residue peptide conserved for all known sequences, binds a phosphate in the active site pocket. His240 is the most likely candidate for the base to oxidize the 1-hydroxyl group of the glucose 6-phosphate substrate.
| + | [[Category: Large Structures]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1DPG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leuconostoc_mesenteroides Leuconostoc mesenteroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DPG OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A resolution., Rowland P, Basak AK, Gover S, Levy HR, Adams MJ, Structure. 1994 Nov 15;2(11):1073-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7881907 7881907]
| + | |
| - | [[Category: Glucose-6-phosphate 1-dehydrogenase]] | + | |
| | [[Category: Leuconostoc mesenteroides]] | | [[Category: Leuconostoc mesenteroides]] |
| - | [[Category: Single protein]]
| + | [[Category: Adams MJ]] |
| - | [[Category: Adams, M J.]] | + | [[Category: Gover S]] |
| - | [[Category: Gover, S.]] | + | [[Category: Rowland P]] |
| - | [[Category: Rowland, P.]] | + | |
| - | [[Category: glucose metabolism]]
| + | |
| - | [[Category: nadp/nad]]
| + | |
| - | [[Category: oxidoreductase]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:46:03 2008''
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