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2uyo

From Proteopedia

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Current revision

Crystal structure of ML2640c from Mycobacterium leprae in an hexagonal crystal form

Structural highlights

2uyo is a 1 chain structure with sequence from Mycobacterium leprae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Y2640_MYCLE Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Mycobacterium leprae protein ML2640c belongs to a large family of conserved hypothetical proteins predominantly found in mycobacteria, some of them predicted as putative S-adenosylmethionine (AdoMet)-dependent methyltransferases (MTase). As part of a Structural Genomics initiative on conserved hypothetical proteins in pathogenic mycobacteria, we have determined the structure of ML2640c in two distinct crystal forms. As expected, ML2640c has a typical MTase core domain and binds the methyl donor substrate AdoMet in a manner consistent with other known members of this structural family. The putative acceptor substrate-binding site of ML2640c is a large internal cavity, mostly lined by aromatic and aliphatic side-chain residues, suggesting that a lipid-like molecule might be targeted for catalysis. A flap segment (residues 222-256), which isolates the binding site from the bulk solvent and is highly mobile in the crystal structures, could serve as a gateway to allow substrate entry and product release. The multiple sequence alignment of ML2640c-like proteins revealed that the central alpha/beta core and the AdoMet-binding site are very well conserved within the family. However, the amino acid positions defining the binding site for the acceptor substrate display a higher variability, suggestive of distinct acceptor substrate specificities. The ML2640c crystal structures offer the first structural glimpses at this important family of mycobacterial proteins and lend strong support to their functional assignment as AdoMet-dependent methyltransferases.

The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria.,Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM. The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria. Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248 doi:10.1110/ps.072982707
↑ Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM. The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria. Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248 doi:10.1110/ps.072982707

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uyo"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF ML2640C FROM MYCOBACTERIUM LEPRAE IN AN HEXAGONAL CRYSTAL FORM==
-<StructureSection load='2uyo' size='340' side='right' caption='[[2uyo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+==Crystal structure of ML2640c from Mycobacterium leprae in an hexagonal crystal form==
+<StructureSection load='2uyo' size='340' side='right'caption='[[2uyo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2uyo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_leprae"_hansen_1880 "bacillus leprae" hansen 1880]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UYO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2uyo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UYO FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ckd|2ckd]], [[2uyq|2uyq]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyo OCA], [http://pdbe.org/2uyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uyo RCSB], [http://www.ebi.ac.uk/pdbsum/2uyo PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyo OCA], [https://pdbe.org/2uyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uyo RCSB], [https://www.ebi.ac.uk/pdbsum/2uyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE]] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref>
+[https://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uy/2uyo_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uy/2uyo_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus leprae hansen 1880]]
+[[Category: Large Structures]]
-[[Category: Alzari, P M]]
+[[Category: Mycobacterium leprae]]
-[[Category: Buschiazzo, A]]
+[[Category: Alzari PM]]
-[[Category: Grana, M]]
+[[Category: Buschiazzo A]]
-[[Category: Haouz, A]]
+[[Category: Grana M]]
-[[Category: Miras, I]]
+[[Category: Haouz A]]
-[[Category: Shepard, W]]
+[[Category: Miras I]]
-[[Category: Wehenkel, A]]
+[[Category: Shepard W]]
-[[Category: Hypothetical protein]]
+[[Category: Wehenkel A]]
-[[Category: Putative methyltransferase]]
-[[Category: Transferase]]

2uyo

From Proteopedia

Current revision

Crystal structure of ML2640c from Mycobacterium leprae in an hexagonal crystal form

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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