1gvz

==PROSTATE SPECIFIC ANTIGEN (PSA) FROM STALLION SEMINAL PLASMA==

<StructureSection load='1gvz' size='340' side='right' caption='[[1gvz]], [[Resolution|resolution]] 1.42&Aring;' scene=''>

<table><tr><td colspan='2'>[[1gvz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GVZ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tissue_kallikrein Tissue kallikrein], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.35 3.4.21.35] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gvz OCA], [http://pdbe.org/1gvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gvz RCSB], [http://www.ebi.ac.uk/pdbsum/1gvz PDBsum]</span></td></tr>

[[http://www.uniprot.org/uniprot/KLK2_HORSE KLK2_HORSE]] Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gv/1gvz_consurf.spt"</scriptWhenChecked>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

Prostate-specific kallikrein, a member of the gene family of serine proteases, was initially discovered in semen and is the most useful serum marker for prostate cancer diagnosis and prognosis. We report the crystal structure at 1.42A resolution of horse prostate kallikrein (HPK). This is the first structure of a serine protease purified from seminal plasma. HPK shares extensive sequence homology with human prostate-specific antigen (PSA), including a predicted chymotrypsin-like specificity, as suggested by the presence of a serine residue at position S1 of the specificity pocket. In contrast to other kallikreins, HPK shows a structurally distinct specificity pocket. Its entrance is blocked by the kallikrein loop, suggesting a possible protective or substrate-selective role for this loop. The HPK structure seems to be in an inactivated state and further processing might be required to allow the binding of substrate molecules. Crystal soaking experiments revealed a binding site for Zn(2+) and Hg(2+), two known PSA inhibitors.

Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA.,Carvalho AL, Sanz L, Barettino D, Romero A, Calvete JJ, Romao MJ J Mol Biol. 2002 Sep 13;322(2):325-37. PMID:12217694<ref>PMID:12217694</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 1gvz" style="background-color:#fffaf0;"></div>

*[[Kallikrein|Kallikrein]]

[[Category: Tissue kallikrein]]

[[Category: Barettino, D]]

[[Category: Calvete, J J]]

[[Category: Carvalho, A L]]

[[Category: Romao, M J]]

[[Category: Romero, A]]

[[Category: Sanz, L]]

[[Category: Prostate specific antigen]]