1e4p
From Proteopedia
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| - | + | ==Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site== | |
| - | + | <StructureSection load='1e4p' size='340' side='right'caption='[[1e4p]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1e4p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora Neurospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E4P FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4p OCA], [https://pdbe.org/1e4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e4p RCSB], [https://www.ebi.ac.uk/pdbsum/1e4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e4p ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | + | == Publication Abstract from PubMed == | |
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The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure. | The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure. | ||
| - | + | Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site.,Michiels PJ, Schouten CH, Hilbers CW, Heus HA RNA. 2000 Dec;6(12):1821-32. PMID:11142381<ref>PMID:11142381</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1e4p" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Neurospora]] | ||
| + | [[Category: Heus HA]] | ||
| + | [[Category: Hilbers CW]] | ||
| + | [[Category: Michiels PJA]] | ||
| + | [[Category: Schouten CHJ]] | ||
Current revision
Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site
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