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3hnu

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Crystal structure of a designed Cyanovirin-N homolog lectin; LKAMG in P21 space group

Structural highlights

3hnu is a 1 chain structure with sequence from Neurospora crassa and Tuber borchii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.56Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CVNH_NEUCR Mannose-binding lectin.^[1] CVNH_TUBBO Mannose-binding lectin.^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The NMR and X-ray structures of a designed chimeric cyanovirin-N homolog (CVNH) protein were determined. The individual halves of the structure are similar to their counterparts in the parent proteins, with domains A and B resembling the structures of TbCVNH and NcCVNH, respectively. No significant differences between the solution and crystal conformations were observed, although details in loop conformations and distinct crystal packing-induced features are present. Carbohydrate binding studies by NMR revealed affinity and specificity for Glc alpha(1-2)Frc and Man alpha(1-2)Man, and the parental half that is devoid of any sucrose affinity in NcCVNH was transformed into a genuine sucrose binding site in the context of the chimera. The atomic details of sugar recognition are seen in the crystal structure of the protein with two bound Glc alpha(1-2)Frc molecules. Both sugars exhibit different conformations around the glycosidic bond and engage in unique hydrogen bonding networks in the two sites. Although the protein is able to bind two Man alpha(1-2)Man molecules, a property associated with HIV-inactivation, no anti-HIV activity was observed for the hybrid protein. These results provide the structural basis for sugar recognition in the CVNH family and aid in deciphering the relationship between sugar binding and anti-HIV activity.

A designed chimeric cyanovirin-N homolog lectin: structure and molecular basis of sucrose binding.,Koharudin LM, Furey W, Gronenborn AM Proteins. 2009 Dec;77(4):904-15. PMID:19639634^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Koharudin LM, Viscomi AR, Jee JG, Ottonello S, Gronenborn AM. The evolutionarily conserved family of cyanovirin-N homologs: structures and carbohydrate specificity. Structure. 2008 Apr;16(4):570-84. PMID:18400178 doi:10.1016/j.str.2008.01.015
↑ Koharudin LM, Viscomi AR, Jee JG, Ottonello S, Gronenborn AM. The evolutionarily conserved family of cyanovirin-N homologs: structures and carbohydrate specificity. Structure. 2008 Apr;16(4):570-84. PMID:18400178 doi:10.1016/j.str.2008.01.015
↑ Koharudin LM, Furey W, Gronenborn AM. A designed chimeric cyanovirin-N homolog lectin: structure and molecular basis of sucrose binding. Proteins. 2009 Dec;77(4):904-15. PMID:19639634 doi:10.1002/prot.22514

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hnu"

@@ Line 1: / Line 1: @@
 ==Crystal structure of a designed Cyanovirin-N homolog lectin; LKAMG in P21 space group==
-<StructureSection load='3hnu' size='340' side='right' caption='[[3hnu]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
+<StructureSection load='3hnu' size='340' side='right'caption='[[3hnu]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3hnu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tubbo Tubbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HNU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3hnu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa] and [https://en.wikipedia.org/wiki/Tuber_borchii Tuber borchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HNU FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kjl|2kjl]], [[3hnx|3hnx]], [[3hp8|3hp8]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hnu OCA], [http://pdbe.org/3hnu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hnu RCSB], [http://www.ebi.ac.uk/pdbsum/3hnu PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hnu OCA], [https://pdbe.org/3hnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hnu RCSB], [https://www.ebi.ac.uk/pdbsum/3hnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hnu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CVNH_TUBBO CVNH_TUBBO]] Mannose-binding lectin.<ref>PMID:18400178</ref>
+[https://www.uniprot.org/uniprot/CVNH_NEUCR CVNH_NEUCR] Mannose-binding lectin.<ref>PMID:18400178</ref> [https://www.uniprot.org/uniprot/CVNH_TUBBO CVNH_TUBBO] Mannose-binding lectin.<ref>PMID:18400178</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/3hnu_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/3hnu_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Tubbo]]
+[[Category: Large Structures]]
-[[Category: Furey, W]]
+[[Category: Neurospora crassa]]
-[[Category: Gronenborn, A M]]
+[[Category: Tuber borchii]]
-[[Category: Koharudin, L M.I]]
+[[Category: Furey W]]
-[[Category: Cvnh]]
+[[Category: Gronenborn AM]]
-[[Category: Cyanovirin-n]]
+[[Category: Koharudin LMI]]
-[[Category: Lectin]]
-[[Category: Sugar binding protein]]

3hnu

From Proteopedia

Current revision

Crystal structure of a designed Cyanovirin-N homolog lectin; LKAMG in P21 space group

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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