1o7i

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of a single stranded DNA binding protein

Structural highlights

1o7i is a 2 chain structure with sequence from Saccharolobus solfataricus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSB_SACS2 Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.

Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.,Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Roberts JA, Bell SD, White MF. An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA. Mol Microbiol. 2003 Apr;48(2):361-71. doi: 10.1046/j.1365-2958.2003.03444.x. PMID:12675797 doi:http://dx.doi.org/10.1046/j.1365-2958.2003.03444.x
↑ Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF. Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein. EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373 doi:10.1093/emboj/cdg272

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1o7i"

Categories: Large Structures | Saccharolobus solfataricus P2 | Kerr ID | Naismith JH

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN==
-<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
+==Crystal structure of a single stranded DNA binding protein==
+<StructureSection load='1o7i' size='340' side='right'caption='[[1o7i]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [https://pdbe.org/1o7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [https://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 27: / Line 31: @@
 ==See Also==
-*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
+*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Sulso]]
+[[Category: Large Structures]]
-[[Category: Kerr, I D]]
+[[Category: Saccharolobus solfataricus P2]]
-[[Category: Naismith, J H]]
+[[Category: Kerr ID]]
-[[Category: Dna-binding protein]]
+[[Category: Naismith JH]]
-[[Category: Ob fold]]
-[[Category: Single stranded dna]]

1o7i

From Proteopedia

Current revision

Crystal structure of a single stranded DNA binding protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox