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| - | ==HIGH-RESOLUTION STRUCTURE OF THE PERIPLASMIC ECTOINE-BINDING PROTEIN FROM TEAABC TRAP-TRANSPORTER OF HALOMONAS ELONGATA== | + | |
| - | <StructureSection load='2vpn' size='340' side='right' caption='[[2vpn]], [[Resolution|resolution]] 1.55Å' scene=''> | + | ==High-resolution structure of the periplasmic ectoine-binding protein from TeaABC TRAP-transporter of Halomonas elongata== |
| | + | <StructureSection load='2vpn' size='340' side='right'caption='[[2vpn]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vpn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33173 Atcc 33173]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vpn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halomonas_elongata Halomonas elongata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VPN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4CS:(4S)-2-METHYL-1,4,5,6-TETRAHYDROPYRIMIDINE-4-CARBOXYLIC+ACID'>4CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vpo|2vpo]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4CS:(4S)-2-METHYL-1,4,5,6-TETRAHYDROPYRIMIDINE-4-CARBOXYLIC+ACID'>4CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpn OCA], [http://pdbe.org/2vpn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vpn RCSB], [http://www.ebi.ac.uk/pdbsum/2vpn PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpn OCA], [https://pdbe.org/2vpn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vpn RCSB], [https://www.ebi.ac.uk/pdbsum/2vpn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TEAA_HALED TEAA_HALED] Part of the tripartite ATP-independent periplasmic (TRAP) transport system TeaABC involved in the uptake of ectoine and hydroxyectoine in response to osmotic upshock. Probably functions as a recovery system for synthesized ectoine that leaks out of the cell. Binds ectoine with high affinity. Affinity for hydroxyectoine is approximately 20-fold lower.<ref>PMID:12003950</ref> <ref>PMID:12076815</ref> <ref>PMID:18702523</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vpn_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vpn_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33173]] | + | [[Category: Halomonas elongata]] |
| - | [[Category: Bienert, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Kuhlmann, S I]] | + | [[Category: Bienert R]] |
| - | [[Category: Kunte, H J]] | + | [[Category: Kuhlmann SI]] |
| - | [[Category: Scheltinga, A C.Terwisscha Van]] | + | [[Category: Kunte HJ]] |
| - | [[Category: Ziegler, C]] | + | [[Category: Terwisscha van Scheltinga AC]] |
| - | [[Category: Ectoine]]
| + | [[Category: Ziegler C]] |
| - | [[Category: Hydroxyectoine]]
| + | |
| - | [[Category: Periplasmic binding protein]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Trap-transporter]]
| + | |
| Structural highlights
Function
TEAA_HALED Part of the tripartite ATP-independent periplasmic (TRAP) transport system TeaABC involved in the uptake of ectoine and hydroxyectoine in response to osmotic upshock. Probably functions as a recovery system for synthesized ectoine that leaks out of the cell. Binds ectoine with high affinity. Affinity for hydroxyectoine is approximately 20-fold lower.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
TeaABC from the moderate halophilic bacterium Halomonas elongata belongs to the tripartite ATP-independent periplasmic transporters (TRAP-T), a family of secondary transporters functioning in conjunction with periplasmic substrate binding proteins. TeaABC facilitates the uptake of the compatible solutes ectoine and hydroxyectoine that are accumulated in the cytoplasm under hyperosmotic stress to protect the cell from dehydration. TeaABC is the only known TRAP-T activated by osmotic stress. Currently, our knowledge on the osmoregulated compatible solute transporter is limited to ABC transporters or conventional secondary transporters. Therefore, this study presents the first detailed analysis of the molecular mechanisms underlying substrate recognition of the substrate binding protein of an osmoregulated TRAP-T. In the present study we were able to demonstrate by isothermal titration calorimetry measurements that TeaA is a high-affinity ectoine binding protein ( K d = 0.19 muM) that also has a significant but somewhat lower affinity to hydroxyectoine ( K d = 3.8 muM). Furthermore, we present the structure of TeaA in complex with ectoine at a resolution of 1.55 A and hydroxyectoine at a resolution of 1.80 A. Analysis of the TeaA binding pocket and comparison of its structure to other compatible solute binding proteins from ABC transporters reveal common principles in compatible solute binding but also significant differences like the solvent-mediated specific binding of ectoine to TeaA.
1.55 A Structure of the Ectoine Binding Protein TeaA of the Osmoregulated TRAP-Transporter TeaABC from Halomonas elongata.,Kuhlmann SI, Terwisscha van Scheltinga AC, Bienert R, Kunte HJ, Ziegler C Biochemistry. 2008 Aug 15. PMID:18702523[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grammann K, Volke A, Kunte HJ. New type of osmoregulated solute transporter identified in halophilic members of the bacteria domain: TRAP transporter TeaABC mediates uptake of ectoine and hydroxyectoine in Halomonas elongata DSM 2581(T). J Bacteriol. 2002 Jun;184(11):3078-85. PMID:12003950 doi:10.1128/JB.184.11.3078-3085.2002
- ↑ Tetsch L, Kunte HJ. The substrate-binding protein TeaA of the osmoregulated ectoine transporter TeaABC from Halomonas elongata: purification and characterization of recombinant TeaA. FEMS Microbiol Lett. 2002 Jun 4;211(2):213-8. PMID:12076815 doi:10.1111/j.1574-6968.2002.tb11227.x
- ↑ Kuhlmann SI, Terwisscha van Scheltinga AC, Bienert R, Kunte HJ, Ziegler C. 1.55 A Structure of the Ectoine Binding Protein TeaA of the Osmoregulated TRAP-Transporter TeaABC from Halomonas elongata. Biochemistry. 2008 Aug 15. PMID:18702523 doi:10.1021/bi8006719
- ↑ Kuhlmann SI, Terwisscha van Scheltinga AC, Bienert R, Kunte HJ, Ziegler C. 1.55 A Structure of the Ectoine Binding Protein TeaA of the Osmoregulated TRAP-Transporter TeaABC from Halomonas elongata. Biochemistry. 2008 Aug 15. PMID:18702523 doi:10.1021/bi8006719
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