1eh5

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[[Image:1eh5.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1 COMPLEXED WITH PALMITATE==
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|PDB= 1eh5 |SIZE=350|CAPTION= <scene name='initialview01'>1eh5</scene>, resolution 2.50&Aring;
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<StructureSection load='1eh5' size='340' side='right'caption='[[1eh5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>
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<table><tr><td colspan='2'>[[1eh5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EH5 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Palmitoyl-protein_hydrolase Palmitoyl-protein hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.22 3.1.2.22] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eh5 OCA], [https://pdbe.org/1eh5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eh5 RCSB], [https://www.ebi.ac.uk/pdbsum/1eh5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eh5 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ei9|1EI9]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eh5 OCA], [http://www.ebi.ac.uk/pdbsum/1eh5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eh5 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PPT1_BOVIN PPT1_BOVIN] Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/1eh5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eh5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mutations in palmitoyl-protein thioesterase 1 (PPT1), a lysosomal enzyme that removes fatty acyl groups from cysteine residues in modified proteins, cause the fatal inherited neurodegenerative disorder infantile neuronal ceroid lipofuscinosis. The accumulation of undigested substrates leads to the formation of neuronal storage bodies that are associated with the clinical symptoms. Less severe forms of PPT1 deficiency have been found recently that are caused by a distinct set of PPT1 mutations, some of which retain a small amount of thioesterase activity. We have determined the crystal structure of PPT1 with and without bound palmitate by using multiwavelength anomalous diffraction phasing. The structure reveals an alpha/beta-hydrolase fold with a catalytic triad composed of Ser115-His289-Asp233 and provides insights into the structural basis for the phenotypes associated with PPT1 mutations.
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'''CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1 COMPLEXED WITH PALMITATE'''
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The crystal structure of palmitoyl protein thioesterase 1 and the molecular basis of infantile neuronal ceroid lipofuscinosis.,Bellizzi JJ 3rd, Widom J, Kemp C, Lu JY, Das AK, Hofmann SL, Clardy J Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4573-8. PMID:10781062<ref>PMID:10781062</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1eh5" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Mutations in palmitoyl-protein thioesterase 1 (PPT1), a lysosomal enzyme that removes fatty acyl groups from cysteine residues in modified proteins, cause the fatal inherited neurodegenerative disorder infantile neuronal ceroid lipofuscinosis. The accumulation of undigested substrates leads to the formation of neuronal storage bodies that are associated with the clinical symptoms. Less severe forms of PPT1 deficiency have been found recently that are caused by a distinct set of PPT1 mutations, some of which retain a small amount of thioesterase activity. We have determined the crystal structure of PPT1 with and without bound palmitate by using multiwavelength anomalous diffraction phasing. The structure reveals an alpha/beta-hydrolase fold with a catalytic triad composed of Ser115-His289-Asp233 and provides insights into the structural basis for the phenotypes associated with PPT1 mutations.
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*[[Thioesterase 3D structures|Thioesterase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1EH5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EH5 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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The crystal structure of palmitoyl protein thioesterase 1 and the molecular basis of infantile neuronal ceroid lipofuscinosis., Bellizzi JJ 3rd, Widom J, Kemp C, Lu JY, Das AK, Hofmann SL, Clardy J, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4573-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10781062 10781062]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Palmitoyl-protein hydrolase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bellizzi III JJ]]
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[[Category: Clardy, J.]]
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[[Category: Clardy J]]
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[[Category: Das, A K.]]
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[[Category: Das AK]]
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[[Category: Hofmann, S L.]]
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[[Category: Hofmann SL]]
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[[Category: III, J J.Bellizzi.]]
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[[Category: Kemp C]]
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[[Category: Kemp, C.]]
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[[Category: Lu JY]]
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[[Category: Lu, J Y.]]
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[[Category: Widom J]]
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[[Category: Widom, J.]]
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[[Category: alpha/beta hydrolase]]
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[[Category: glycoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:02:34 2008''
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Current revision

CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1 COMPLEXED WITH PALMITATE

PDB ID 1eh5

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