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1ehw

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[[Image:1ehw.jpg|left|200px]]
 
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{{Structure
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==HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4==
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|PDB= 1ehw |SIZE=350|CAPTION= <scene name='initialview01'>1ehw</scene>, resolution 2.40&Aring;
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<StructureSection load='1ehw' size='340' side='right'caption='[[1ehw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1ehw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EHW FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ehw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ehw OCA], [https://pdbe.org/1ehw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ehw RCSB], [https://www.ebi.ac.uk/pdbsum/1ehw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ehw ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ehw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ehw OCA], [http://www.ebi.ac.uk/pdbsum/1ehw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ehw RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/NDKM_HUMAN NDKM_HUMAN] Major role in the synthesis of nucleoside triphosphates other than ATP (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/1ehw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ehw ConSurf].
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<div style="clear:both"></div>
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'''HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4'''
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==See Also==
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*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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We demonstrate here the catalytic activity and subcellular localization of the Nm23-H4 protein, product of nm23-H4, a new member of the human nm23/nucleoside diphosphate (NDP) kinase gene family (Milon, L., Rousseau-Merck, M., Munier, A., Erent, M., Lascu, I., Capeau, J., and Lacombe, M. L. (1997) Hum. Genet. 99, 550-557). Nm3-H4 was synthesized in escherichia coli as the full-length protein and as a truncated form missing the N-terminal extension characteristic of mitochondrial targeting. The truncated form possesses NDP kinase activity, whereas the full-length protein is inactive, suggesting that the extension prevents enzyme folding and/or activity. X-ray crystallographic analysis was performed on active truncated Nm23-H4. Like other eukaryotic NDP kinases, it is a hexamer. Nm23-H4 naturally possesses a serine residue at position 129, equivalent to the K-pn mutation of the Drosophila NDP kinase. The x-ray structure shows that the presence of Ser(129) has local structural effects that weaken subunit interactions. Site-directed mutagenesis shows that the serine is responsible for the lability of Nm23-H4 to heat and urea treatment, because the S129P mutant is greatly stabilized. Examination of human embryonic kidney 293 cells transfected with green fluorescent protein fusions by confocal microscopy shows a specific mitochondrial localization of Nm23-H4 that was also demonstrated by Western blot analysis of subcellular fractions of these cells. Import into mitochondria is accompanied by cleavage of the N-terminal extension that results in NDP kinase activity. Submitochondrial fractionation indicates that Nm23-H4 is associated with mitochondrial membranes, possibly to the contact sites between the outer and inner membranes.
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==About this Structure==
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1EHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHW OCA].
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==Reference==
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The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase., Milon L, Meyer P, Chiadmi M, Munier A, Johansson M, Karlsson A, Lascu I, Capeau J, Janin J, Lacombe ML, J Biol Chem. 2000 May 12;275(19):14264-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10799505 10799505]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Nucleoside-diphosphate kinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Capeau J]]
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[[Category: Capeau, J.]]
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[[Category: Chiadmi M]]
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[[Category: Chiadmi, M.]]
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[[Category: Janin J]]
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[[Category: Janin, J.]]
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[[Category: Johansson M]]
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[[Category: Johansson, M.]]
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[[Category: Karlsson A]]
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[[Category: Karlsson, A.]]
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[[Category: Lacombe M-L]]
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[[Category: Lacombe, M-L.]]
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[[Category: Lascu I]]
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[[Category: Lascu, I.]]
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[[Category: Meyer P]]
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[[Category: Meyer, P.]]
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[[Category: Milon L]]
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[[Category: Milon, L.]]
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[[Category: Munier A]]
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[[Category: Munier, A.]]
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[[Category: killer-of-prune]]
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[[Category: mitochondrial]]
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[[Category: nm23]]
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[[Category: nucleoside diphosphate kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:02:50 2008''
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Current revision

HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4

PDB ID 1ehw

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