|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | ==STRUCTURE OF BMORI GOBP2 (GENERAL ODORANT BINDING PROTEIN 2) WITH BOMBYKAL== | + | |
| - | <StructureSection load='2wch' size='340' side='right' caption='[[2wch]], [[Resolution|resolution]] 1.70Å' scene=''> | + | ==Structure of BMori GOBP2 (General Odorant Binding Protein 2) with bombykal== |
| | + | <StructureSection load='2wch' size='340' side='right'caption='[[2wch]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2wch]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WCH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WCH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2wch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WCH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B7M:(10E,12Z)-HEXADECA-10,12-DIENAL'>B7M</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wcl|2wcl]], [[2wc6|2wc6]], [[2wck|2wck]], [[2wcm|2wcm]], [[2wc5|2wc5]], [[2wcj|2wcj]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B7M:(10E,12Z)-HEXADECA-10,12-DIENAL'>B7M</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wch OCA], [http://pdbe.org/2wch PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wch RCSB], [http://www.ebi.ac.uk/pdbsum/2wch PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wch OCA], [https://pdbe.org/2wch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wch RCSB], [https://www.ebi.ac.uk/pdbsum/2wch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wch ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/OBP1_BOMMO OBP1_BOMMO]] Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid. | + | [https://www.uniprot.org/uniprot/OBP2_BOMMO OBP2_BOMMO] Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wc/2wch_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wc/2wch_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| Line 35: |
Line 36: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bommo]] | + | [[Category: Bombyx mori]] |
| - | [[Category: Field, L M]] | + | [[Category: Large Structures]] |
| - | [[Category: He, X]] | + | [[Category: Field LM]] |
| - | [[Category: Keep, N H]] | + | [[Category: He X]] |
| - | [[Category: Pickett, J A]] | + | [[Category: Keep NH]] |
| - | [[Category: Robertson, G]] | + | [[Category: Pickett JA]] |
| - | [[Category: Zhou, J J]] | + | [[Category: Robertson G]] |
| - | [[Category: Disulfide bond]]
| + | [[Category: Zhou J-J]] |
| - | [[Category: Insect pheremone]]
| + | |
| - | [[Category: Odorant-binding protein]]
| + | |
| - | [[Category: Olfaction]]
| + | |
| - | [[Category: Sensory transduction]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
OBP2_BOMMO Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla. In the silkworm Bombyx mori, the OBPs are subdivided into three main subfamilies; pheromone-binding proteins (PBPs), general odorant-binding proteins (GOBPs) and antennal-binding proteins (ABPs). We used the MotifSearch algorithm to search for genes encoding putative OBPs in B. mori and found 13, many fewer than are found in the genomes of fruit flies and mosquitoes. The 13 genes include seven new ABP-like OBPs as well as the previously identified PBPs (three), GOBPs (two) and ABPx. Quantitative examination of transcript levels showed that BmorPBP1, BmorGOBP1, BmorGOBP2 and BmorABPx are expressed at very high levels in the antennae and so could be involved in olfaction. A new two-phase binding assay, along with other established assays, showed that BmorPBP1, BmorPBP2, BmorGOBP2 and BmorABPx all bind to the B. mori sex pheromone component (10E,12Z)-hexadecadien-1-ol (bombykol). BmorPBP1, BmorPBP2 and BmorABPx also bind the pheromone component (10E,12Z)-hexadecadienal (bombykal) equally well, whereas BmorGOBP2 can discriminate between bombykol and bombykal. X-ray structures show that when bombykol is bound to BmorGOBP2 it adopts a different conformation from that found when it binds to BmorPBP1. Binding to BmorGOBP2 involves hydrogen bonding to Arg110 rather than to Ser56 as found for BmorPBP1.
Characterisation of Bombyx mori Odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components.,Zhou JJ, Robertson G, He X, Dufour S, Hooper AM, Pickett JA, Keep NH, Field LM J Mol Biol. 2009 Jun 12;389(3):529-45. Epub 2009 Apr 14. PMID:19371749[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhou JJ, Robertson G, He X, Dufour S, Hooper AM, Pickett JA, Keep NH, Field LM. Characterisation of Bombyx mori Odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components. J Mol Biol. 2009 Jun 12;389(3):529-45. Epub 2009 Apr 14. PMID:19371749 doi:10.1016/j.jmb.2009.04.015
|
