1eoe

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[[Image:1eoe.jpg|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN==
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|PDB= 1eoe |SIZE=350|CAPTION= <scene name='initialview01'>1eoe</scene>, resolution 1.704&Aring;
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<StructureSection load='1eoe' size='340' side='right'caption='[[1eoe]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1eoe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOE FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.704&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoe OCA], [https://pdbe.org/1eoe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eoe RCSB], [https://www.ebi.ac.uk/pdbsum/1eoe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoe ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1eod|1EOD]], [[1eof|1EOF]], [[1t1d|1T1D]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoe OCA], [http://www.ebi.ac.uk/pdbsum/1eoe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eoe RCSB]</span>
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[https://www.uniprot.org/uniprot/Q16968_APLCA Q16968_APLCA]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eoe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eoe ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN'''
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==See Also==
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The T1 domain, a highly conserved cytoplasmic portion at the N-terminus of the voltage-dependent K+ channel (Kv) alpha-subunit, is responsible for driving and regulating the tetramerization of the alpha-subunits. Here we report the identification of a set of mutations in the T1 domain that alter the gating properties of the Kv channel. Two mutants produce a leftward shift in the activation curve and slow the channel closing rate while a third mutation produces a rightward shift in the activation curve and speeds the channel closing rate. We have determined the crystal structures of T1 domains containing these mutations. Both of the leftward shifting mutants produce similar conformational changes in the putative membrane facing surface of the T1 domain. These results suggest that the structure of the T1 domain in this region is tightly coupled to the channel's gating states.
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==About this Structure==
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1EOE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOE OCA].
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==Reference==
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Voltage dependent activation of potassium channels is coupled to T1 domain structure., Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, Pfaffinger PJ, Nat Struct Biol. 2000 May;7(5):403-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10802739 10802739]
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Choe, S.]]
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[[Category: Choe S]]
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[[Category: Cushman, S J.]]
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[[Category: Cushman SJ]]
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[[Category: DeRubeis, D.]]
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[[Category: DeRubeis D]]
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[[Category: Jahng, A W.]]
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[[Category: Jahng AW]]
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[[Category: Nanao, M H.]]
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[[Category: Nanao MH]]
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[[Category: Pfaffinger, P J.]]
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[[Category: Pfaffinger PJ]]
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[[Category: aplysia kv1 1]]
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[[Category: potassium channel]]
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[[Category: proton transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:06:41 2008''
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Current revision

CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN

PDB ID 1eoe

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