This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1eoo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:05, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1eoo.gif|left|200px]]
 
-
{{Structure
+
==ECORV BOUND TO COGNATE DNA==
-
|PDB= 1eoo |SIZE=350|CAPTION= <scene name='initialview01'>1eoo</scene>, resolution 2.16&Aring;
+
<StructureSection load='1eoo' size='340' side='right'caption='[[1eoo]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+
<table><tr><td colspan='2'>[[1eoo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOO FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoo OCA], [https://pdbe.org/1eoo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eoo RCSB], [https://www.ebi.ac.uk/pdbsum/1eoo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoo ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoo OCA], [http://www.ebi.ac.uk/pdbsum/1eoo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eoo RCSB]</span>
+
[https://www.uniprot.org/uniprot/T2E5_ECOLX T2E5_ECOLX] Recognizes the double-stranded sequence GATATC and cleaves after T-3.
-
}}
+
-
'''ECORV BOUND TO COGNATE DNA'''
+
==See Also==
-
 
+
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Two new high-resolution cocrystal structures of EcoRV endonuclease bound to DNA show that a large variation in DNA-bending angles is sampled in the ground state binary complex. Together with previous structures, these data reveal a contiguous series of protein conformational states delineating a specific trajectory for the induced-fit pathway. Rotation of the DNA-binding domains, together with movements of two symmetry-related helices binding in the minor groove, causes base unstacking at a key base-pair step and propagates structural changes that assemble the active sites. These structures suggest a complex mechanism for DNA bending that depends on forces generated by interacting protein segments, and on selective neutralization of phosphate charges along the inner face of the bent double helix.
+
-
 
+
-
==About this Structure==
+
-
1EOO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOO OCA].
+
-
 
+
-
==Reference==
+
-
Crystallographic snapshots along a protein-induced DNA-bending pathway., Horton NC, Perona JJ, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5729-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10801972 10801972]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Type II site-specific deoxyribonuclease]]
+
[[Category: Horton NC]]
-
[[Category: Horton, N C.]]
+
[[Category: Perona JJ]]
-
[[Category: Perona, J J.]]
+
-
[[Category: endonuclease]]
+
-
[[Category: induced fit]]
+
-
[[Category: protein-dna recognition]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:06:45 2008''
+

Current revision

ECORV BOUND TO COGNATE DNA

PDB ID 1eoo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1eoo"
Personal tools