2c0d

From Proteopedia

(Difference between revisions)

Current revision

Structure of the mitochondrial 2-cys peroxiredoxin from Plasmodium falciparum

Structural highlights

2c0d is a 2 chain structure with sequence from Plasmodium falciparum 3D7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.78Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8I5Q6_PLAF7 Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.[PIRNR:PIRNR000239]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Plasmodium falciparum possesses a single mitochondrion with a functional electron transport chain. During respiration, reactive oxygen species are generated that need to be removed to protect the organelle from oxidative damage. In the absence of catalase and glutathione peroxidase, the parasites rely primarily on peroxiredoxin-linked systems for protection. We have analysed the biochemical and structural features of the mitochondrial peroxiredoxin and thioredoxin of P. falciparum. The mitochondrial localization of both proteins was confirmed by expressing green fluorescent protein fusions in parasite erythrocytic stages. Recombinant protein was kinetically characterized using the cytosolic and the mitochondrial thioredoxin (PfTrx1 and PfTrx2 respectively). The peroxiredoxin clearly preferred PfTrx2 to PfTrx1 as a reducing partner, reflected by the KM values of 11.6 microM and 130.4 microM respectively. Substitution of the two dyads asparagine-62/tyrosine-63 and phenylalanine-139/alanine-140 residues by aspartate-phenylalaine and valine-serine, respectively, reduced the KM for Trx1 but had no effect on the KM of Trx2 suggesting some role for these residues in the discrimination between the two substrates. Solution studies suggest that the protein exists primarily in a homodecameric form. The crystal structure of the mitochondrial peroxiredoxin reveals a fold typical of the 2-Cys class peroxiredoxins and a dimeric form with an intermolecular disulphide bridge between Cys67 and Cys187. These results show that the mitochondrial peroxiredoxin of P. falciparum occurs in both dimeric and decameric forms when purified under non-reducing conditions.

Structural and biochemical characterization of a mitochondrial peroxiredoxin from Plasmodium falciparum.,Boucher IW, McMillan PJ, Gabrielsen M, Akerman SE, Brannigan JA, Schnick C, Brzozowski AM, Wilkinson AJ, Muller S Mol Microbiol. 2006 Aug;61(4):948-59. PMID:16879648^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Boucher IW, McMillan PJ, Gabrielsen M, Akerman SE, Brannigan JA, Schnick C, Brzozowski AM, Wilkinson AJ, Muller S. Structural and biochemical characterization of a mitochondrial peroxiredoxin from Plasmodium falciparum. Mol Microbiol. 2006 Aug;61(4):948-59. PMID:16879648 doi:10.1111/j.1365-2958.2006.05303.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c0d"

@@ Line 1: / Line 1: @@
-==STRUCTURE OF THE MITOCHONDRIAL 2-CYS PEROXIREDOXIN FROM PLASMODIUM FALCIPARUM==
-<StructureSection load='2c0d' size='340' side='right' caption='[[2c0d]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
+==Structure of the mitochondrial 2-cys peroxiredoxin from Plasmodium falciparum==
+<StructureSection load='2c0d' size='340' side='right'caption='[[2c0d]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2c0d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C0D FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2c0d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C0D FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0d OCA], [http://pdbe.org/2c0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c0d RCSB], [http://www.ebi.ac.uk/pdbsum/2c0d PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0d OCA], [https://pdbe.org/2c0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c0d RCSB], [https://www.ebi.ac.uk/pdbsum/2c0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c0d ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8I5Q6_PLAF7 Q8I5Q6_PLAF7] Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.[PIRNR:PIRNR000239]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/2c0d_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/2c0d_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 27: / Line 30: @@
 ==See Also==
-*[[Peroxiredoxin|Peroxiredoxin]]
+*[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Peroxidase]]
+[[Category: Large Structures]]
-[[Category: Plaf7]]
+[[Category: Plasmodium falciparum 3D7]]
-[[Category: Boucher, I W]]
+[[Category: Boucher IW]]
-[[Category: Brannigan, J A]]
+[[Category: Brannigan JA]]
-[[Category: Brzozowski, A M]]
+[[Category: Brzozowski AM]]
-[[Category: Muller, S]]
+[[Category: Muller S]]
-[[Category: Wilkinson, A J]]
+[[Category: Wilkinson AJ]]
-[[Category: 2-cy]]
-[[Category: Antioxidant]]
-[[Category: Mitochondrial]]
-[[Category: Oxidoreductase]]
-[[Category: Peroxiredoxin]]
-[[Category: Redox-active center]]
-[[Category: Thioredoxin dependant]]

2c0d

From Proteopedia

Current revision

Structure of the mitochondrial 2-cys peroxiredoxin from Plasmodium falciparum

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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