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1w4z

From Proteopedia

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Current revision

Structure of actinorhodin polyketide (actIII) Reductase

Structural highlights

1w4z is a 2 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ACT3_STRCO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have determined the 2.5 angstroms crystal structure of an active, tetrameric Streptomyces coelicolor type II polyketide ketoreductase (actIII) with its bound cofactor, NADP+. This structure shows a Rossman dinucleotide binding fold characteristic of SDR enzymes. Of two subunits in the crystallographic asymmetric unit, one is closed around the active site. Formate is observed in the open subunit, indicating possible carbonyl binding sites of the polyketide intermediate. Unlike previous models we observe crystal contacts that may mimic the KR-ACP interactions that may drive active site opening. Based on these observations, we have constructed a model for ACP and polyketide binding. We propose that binding of ACP triggers a conformational change from the closed to the open, active form of the enzyme. The polyketide chain enters the active site and reduction occurs. The model also suggests a general mechanism for ACP recognition which is applicable to a range of protein families.

The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding.,Hadfield AT, Limpkin C, Teartasin W, Simpson TJ, Crosby J, Crump MP Structure. 2004 Oct;12(10):1865-75. PMID:15458634^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hadfield AT, Limpkin C, Teartasin W, Simpson TJ, Crosby J, Crump MP. The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding. Structure. 2004 Oct;12(10):1865-75. PMID:15458634 doi:10.1016/j.str.2004.08.002

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w4z"

@@ Line 1: / Line 1: @@
-==STRUCTURE OF ACTINORHODIN POLYKETIDE (ACTIII) REDUCTASE==
-<StructureSection load='1w4z' size='340' side='right' caption='[[1w4z]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+==Structure of actinorhodin polyketide (actIII) Reductase==
+<StructureSection load='1w4z' size='340' side='right'caption='[[1w4z]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1w4z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W4Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1w4z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W4Z FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w4z OCA], [http://pdbe.org/1w4z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w4z RCSB], [http://www.ebi.ac.uk/pdbsum/1w4z PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w4z OCA], [https://pdbe.org/1w4z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w4z RCSB], [https://www.ebi.ac.uk/pdbsum/1w4z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w4z ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/ACT3_STRCO ACT3_STRCO]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/1w4z_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/1w4z_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Crosby, J]]
+[[Category: Large Structures]]
-[[Category: Crump, M P]]
+[[Category: Streptomyces coelicolor]]
-[[Category: Hadfield, A T]]
+[[Category: Crosby J]]
-[[Category: Limpkin, C]]
+[[Category: Crump MP]]
-[[Category: Simpson, T J]]
+[[Category: Hadfield AT]]
-[[Category: Teartasin, W]]
+[[Category: Limpkin C]]
-[[Category: Acp binding]]
+[[Category: Simpson TJ]]
-[[Category: Antibiotic biosynthesis]]
+[[Category: Teartasin W]]
-[[Category: Ketoreductase]]
-[[Category: Sdr]]
-[[Category: Type ii polyketide synthesis]]

1w4z

From Proteopedia

Current revision

Structure of actinorhodin polyketide (actIII) Reductase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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