1esh
From Proteopedia
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| - | [[Image:1esh.gif|left|200px]] | ||
| - | + | ==THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA== | |
| - | + | <StructureSection load='1esh' size='340' side='right'caption='[[1esh]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1esh]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESH FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esh OCA], [https://pdbe.org/1esh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esh RCSB], [https://www.ebi.ac.uk/pdbsum/1esh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esh ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | + | == Publication Abstract from PubMed == | |
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The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that directs its RNA synthesis. A stem loop structure in this sequence, stem loop C (SLC), was investigated using NMR, and correlated with its ability to direct RNA synthesis by its replicase. SLC consists of two discrete domains, a flexible stem with an internal loop and a rigid stem containing a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only one side of the flexible stem and a specific compact conformation of the triloop. A high resolution structure of the triloop places the 5' adenine out in solution, and the 3' adenine within the triloop, held tightly through stacking and unusual hydrogen bonds. This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis. | The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that directs its RNA synthesis. A stem loop structure in this sequence, stem loop C (SLC), was investigated using NMR, and correlated with its ability to direct RNA synthesis by its replicase. SLC consists of two discrete domains, a flexible stem with an internal loop and a rigid stem containing a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only one side of the flexible stem and a specific compact conformation of the triloop. A high resolution structure of the triloop places the 5' adenine out in solution, and the 3' adenine within the triloop, held tightly through stacking and unusual hydrogen bonds. This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis. | ||
| - | + | RNA motifs that determine specificity between a viral replicase and its promoter.,Kim CH, Kao CC, Tinoco I Jr Nat Struct Biol. 2000 May;7(5):415-23. PMID:10802741<ref>PMID:10802741</ref> | |
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| - | RNA motifs that determine specificity between a viral replicase and its promoter., Kim CH, Kao CC, Tinoco I Jr | + | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 1esh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kao CC]] | ||
| + | [[Category: Kim C-H]] | ||
| + | [[Category: Tinoco Jr I]] | ||
Current revision
THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA
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