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3gyj

From Proteopedia

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==Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A)==
==Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A)==
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<StructureSection load='3gyj' size='340' side='right' caption='[[3gyj]], [[Resolution|resolution]] 0.92&Aring;' scene=''>
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<StructureSection load='3gyj' size='340' side='right'caption='[[3gyj]], [[Resolution|resolution]] 0.92&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3gyj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strs0 Strs0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GYJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3gyj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SA-COO Streptomyces sp. SA-COO]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GYJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.92&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mxt|1mxt]], [[1ijh|1ijh]], [[3gyi|3gyi]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">choA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=74576 STRS0])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gyj OCA], [https://pdbe.org/3gyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gyj RCSB], [https://www.ebi.ac.uk/pdbsum/3gyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gyj ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gyj OCA], [http://pdbe.org/3gyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gyj RCSB], [http://www.ebi.ac.uk/pdbsum/3gyj PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CHOD_STRS0 CHOD_STRS0]] Bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product.
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[https://www.uniprot.org/uniprot/CHOD_STRS0 CHOD_STRS0] Bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gy/3gyj_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gy/3gyj_consurf.spt"</scriptWhenChecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cholesterol oxidase]]
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[[Category: Large Structures]]
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[[Category: Strs0]]
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[[Category: Streptomyces sp. SA-COO]]
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[[Category: Lyubimov, A Y]]
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[[Category: Lyubimov AY]]
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[[Category: Vrielink, A]]
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[[Category: Vrielink A]]
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[[Category: Cholesterol metabolism]]
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[[Category: Fad]]
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[[Category: Flavoprotein]]
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[[Category: Gmc oxidoreductase]]
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[[Category: Hydrophobic tunnel]]
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[[Category: Lipid metabolism]]
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[[Category: Oxidoreductase]]
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[[Category: Secreted]]
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[[Category: Site-directed mutagenesis]]
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[[Category: Steroid metabolism]]
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Current revision

Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A)

PDB ID 3gyj

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