2vyw

From Proteopedia

(Difference between revisions)

Current revision

Hemoglobin (Hb2) from trematode Fasciola hepatica

Structural highlights

2vyw is a 1 chain structure with sequence from Fasciola hepatica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The trematode Fasciola hepatica (Fa.he.) is a common parasite of human and livestock. The hemoglobin (Hb) of Fa.he., a potential immunogen, was chosen for characterization in the search for an effective vaccine. Characterization of trematode Hbs show that they are intracellular single-domain globins with the following remarkable features: (1) Fa.he. expresses two Hb isoforms that differ at two amino acid sites (F1: 119Y/123Q; F2: 119F/123L). Both isoforms are monoacetylated at their N-termini; (2) the genes coding for Fa.he. and Paramphistomum epiclitum (Pa.ep.) Hbs are interrupted by two introns at the conserved positions B12.2 and G7.0.; (3) UV/VIS and resonance Raman spectroscopy identify the recombinant Fa.he. HbF2 as a pentacoordinated high-spin ferrous Hb; (4) electron paramagnetic resonance spectroscopy of cyano-met Fa.he. HbF2 proves that the endogenously bound imidazole has no imidazolate character; (5) the major structural determinants of the globin fold are present, they contain a TyrB10/TyrE7 residue pair on the distal side. Although such distal-site pair is a signature for high oxygen affinity, as shown for Pa.ep. Hb, the oxygen-binding rate parameters for Fa.he. Hb are intermediate between those of myoglobin and those of other trematode Hbs; (6) the three-dimensional structure of recombinant Fa.he. HbF2 from this study closely resembles the three-dimensional structure of Pa.ep. determined earlier. The set of distal-site polar interactions observed in Pa.ep. Hb is matched with small but significant structural adjustments; (7) despite the potential immunogenic character of the fluke Hb, vaccination of calves with recombinant Fa.he. HbF2 failed to promote protection against parasitic infection.

The hemoglobins of the trematodes Fasciola hepatica and Paramphistomum epiclitum: a molecular biological, physico-chemical, kinetic, and vaccination study.,Dewilde S, Ioanitescu AI, Kiger L, Gilany K, Marden MC, Van Doorslaer S, Vercruysse J, Pesce A, Nardini M, Bolognesi M, Moens L Protein Sci. 2008 Oct;17(10):1653-62. Epub 2008 Jul 11. PMID:18621914^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dewilde S, Ioanitescu AI, Kiger L, Gilany K, Marden MC, Van Doorslaer S, Vercruysse J, Pesce A, Nardini M, Bolognesi M, Moens L. The hemoglobins of the trematodes Fasciola hepatica and Paramphistomum epiclitum: a molecular biological, physico-chemical, kinetic, and vaccination study. Protein Sci. 2008 Oct;17(10):1653-62. Epub 2008 Jul 11. PMID:18621914 doi:10.1110/ps.036558.108

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vyw"

@@ Line 1: / Line 1: @@
-==HEMOGLOBIN (HB2) FROM TREMATODE FASCIOLA HEPATICA==
-<StructureSection load='2vyw' size='340' side='right' caption='[[2vyw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+==Hemoglobin (Hb2) from trematode Fasciola hepatica==
+<StructureSection load='2vyw' size='340' side='right'caption='[[2vyw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vyw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fasciola_hepatica Fasciola hepatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VYW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fasciola_hepatica Fasciola hepatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VYW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vyw OCA], [http://pdbe.org/2vyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vyw RCSB], [http://www.ebi.ac.uk/pdbsum/2vyw PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vyw OCA], [https://pdbe.org/2vyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vyw RCSB], [https://www.ebi.ac.uk/pdbsum/2vyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vyw ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 10: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/2vyw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/2vyw_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 33: / Line 35: @@
 </StructureSection>
 [[Category: Fasciola hepatica]]
-[[Category: Bolognesi, M]]
+[[Category: Large Structures]]
-[[Category: Dewilde, S]]
+[[Category: Bolognesi M]]
-[[Category: Doorslaer, S Van]]
+[[Category: Dewilde S]]
-[[Category: Gilany, K]]
+[[Category: Gilany K]]
-[[Category: Ioanitescu, A I]]
+[[Category: Ioanitescu AI]]
-[[Category: Kiger, L]]
+[[Category: Kiger L]]
-[[Category: Marden, M C]]
+[[Category: Marden MC]]
-[[Category: Moens, L]]
+[[Category: Moens L]]
-[[Category: Nardini, M]]
+[[Category: Nardini M]]
-[[Category: Pesce, A]]
+[[Category: Pesce A]]
-[[Category: Vercruysse, J]]
+[[Category: Van Doorslaer S]]
-[[Category: Hemoglobin]]
+[[Category: Vercruysse J]]
-[[Category: Oxygen binding]]
-[[Category: Trematode]]

2vyw

From Proteopedia

Current revision

Hemoglobin (Hb2) from trematode Fasciola hepatica

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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