1f0c

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[[Image:1f0c.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF THE VIRAL SERPIN CRMA==
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|PDB= 1f0c |SIZE=350|CAPTION= <scene name='initialview01'>1f0c</scene>, resolution 2.26&Aring;
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<StructureSection load='1f0c' size='340' side='right'caption='[[1f0c]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>
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<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [https://pdbe.org/1f0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [https://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f0c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f0c ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF THE VIRAL SERPIN CRMA'''
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==See Also==
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*[[Serpin 3D structures|Serpin 3D structures]]
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== References ==
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==Overview==
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<references/>
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BACKGROUND: Cowpox virus expresses the serpin CrmA (cytokine response modifier A) in order to avoid inflammatory and apoptotic responses of infected host cells. The targets of CrmA are members of the caspase family of proteases that either initiate the extrinsic pathway of apoptosis (caspases 8 and 10) or trigger activation of the pro-inflammatory cytokines interleukin-1beta and interleukin-18 (caspase 1). RESULTS: We have determined the structure of a cleaved form of CrmA to 2.26 A resolution. CrmA has the typical fold of a cleaved serpin, even though it lacks the N-terminal half of the A helix, the entire D helix, and a portion of the E helix that are present in all other known serpins. The reactive-site loop of CrmA was mutated to contain the optimal substrate recognition sequence for caspase 3; however, the mutation only marginally increased the ability of CrmA to inhibit caspase 3. Superposition of the reactive-site loop of alpha1-proteinase inhibitor on the cleaved CrmA structure provides a model for virgin CrmA that can be docked to caspase 1, but not to caspase 3. CONCLUSIONS: CrmA exemplifies viral economy, selective pressure having resulted in a 'minimal' serpin that lacks the regions not needed for structural integrity or inhibitory activity. The docking model provides an explanation for the selectivity of CrmA. Our demonstration that engineering optimal substrate recognition sequences into the CrmA reactive-site loop fails to generate a good caspase 3 inhibitor is consistent with the docking model.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Cowpox virus]]
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1F0C is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Cow_pox_virus Cow pox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA].
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[[Category: Large Structures]]
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[[Category: Bankston LA]]
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==Reference==
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[[Category: Liddington RC]]
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Crystal structure of the apoptotic suppressor CrmA in its cleaved form., Renatus M, Zhou Q, Stennicke HR, Snipas SJ, Turk D, Bankston LA, Liddington RC, Salvesen GS, Structure. 2000 Jul 15;8(7):789-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10903953 10903953]
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[[Category: Renatus M]]
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[[Category: Cow pox virus]]
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[[Category: Salvesen GS]]
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[[Category: Protein complex]]
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[[Category: Snipas SJ]]
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[[Category: Bankston, L A.]]
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[[Category: Stennicke HR]]
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[[Category: Liddington, R C.]]
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[[Category: Turk D]]
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[[Category: Renatus, M.]]
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[[Category: Zhou Q]]
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[[Category: Salvesen, G S.]]
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[[Category: Snipas, S J.]]
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[[Category: Stennicke, H R.]]
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[[Category: Turk, D.]]
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[[Category: Zhou, Q.]]
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[[Category: apoptosis]]
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[[Category: caspase inhibitor]]
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[[Category: protease inhibitor]]
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[[Category: serpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:13:15 2008''
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Current revision

STRUCTURE OF THE VIRAL SERPIN CRMA

PDB ID 1f0c

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