2jnf

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of fly troponin C, isoform F1

Structural highlights

2jnf is a 1 chain structure with sequence from Lethocerus indicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q868D4_9HEMI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.

The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles.,De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A Structure. 2007 Jul;15(7):813-24. PMID:17637342^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A. The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles. Structure. 2007 Jul;15(7):813-24. PMID:17637342 doi:10.1016/j.str.2007.05.007

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jnf"

Categories: Large Structures | Lethocerus indicus | Bullard B | De Nicola GF | Pastore A

@@ Line 1: / Line 1: @@
 ==Solution structure of fly troponin C, isoform F1==
-<StructureSection load='2jnf' size='340' side='right' caption='[[2jnf]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2jnf' size='340' side='right'caption='[[2jnf]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lethocerus_indica Lethocerus indica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JNF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JNF FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212017 Lethocerus indica])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [http://pdbe.org/2jnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB], [http://www.ebi.ac.uk/pdbsum/2jnf PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [https://pdbe.org/2jnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB], [https://www.ebi.ac.uk/pdbsum/2jnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jnf ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q868D4_9HEMI Q868D4_9HEMI]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/2jnf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/2jnf_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 27: / Line 30: @@
 ==See Also==
-*[[Troponin|Troponin]]
+*[[Troponin 3D structures|Troponin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Lethocerus indica]]
+[[Category: Large Structures]]
-[[Category: Bullard, B]]
-[[Category: Nicola, G F.De]]
-[[Category: Pastore, A]]
-[[Category: Ef-hand]]
 [[Category: Lethocerus indicus]]
-[[Category: Metal binding protein]]
+[[Category: Bullard B]]
-[[Category: Stretch activated muscle contraction]]
+[[Category: De Nicola GF]]
-[[Category: Troponin c]]
+[[Category: Pastore A]]

2jnf

From Proteopedia

Current revision

Solution structure of fly troponin C, isoform F1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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