1wsi
From Proteopedia
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==Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N)== | ==Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N)== | ||
| - | <StructureSection load='1wsi' size='340' side='right' caption='[[1wsi]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='1wsi' size='340' side='right'caption='[[1wsi]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1wsi]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1wsi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WSI FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsi OCA], [https://pdbe.org/1wsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wsi RCSB], [https://www.ebi.ac.uk/pdbsum/1wsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsi ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/RNH_ECOLI RNH_ECOLI] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.[HAMAP-Rule:MF_00042] |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1wsi_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1wsi_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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==See Also== | ==See Also== | ||
| - | *[[Ribonuclease|Ribonuclease | + | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
| - | + | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Escherichia coli]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Kanaya | + | [[Category: Kanaya S]] |
| - | [[Category: Matsumura | + | [[Category: Matsumura H]] |
| - | [[Category: Takano | + | [[Category: Takano K]] |
| - | [[Category: Tsunaka | + | [[Category: Tsunaka Y]] |
| - | [[Category: Yamagata | + | [[Category: Yamagata Y]] |
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Current revision
Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N)
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