1f4u

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[[Image:1f4u.jpg|left|200px]]
 
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{{Structure
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==THERMOPHILIC P450: CYP119 FROM SULFOLOBUS SOLFACTARICUS==
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|PDB= 1f4u |SIZE=350|CAPTION= <scene name='initialview01'>1f4u</scene>, resolution 2.69&Aring;
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<StructureSection load='1f4u' size='340' side='right'caption='[[1f4u]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1f4u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F4U FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4u OCA], [https://pdbe.org/1f4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f4u RCSB], [https://www.ebi.ac.uk/pdbsum/1f4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f4u ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1f4t|1F4T]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4u OCA], [http://www.ebi.ac.uk/pdbsum/1f4u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f4u RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CP119_SULAC CP119_SULAC] The endogenous substrate is not known. In vitro, catalyzes the H(2)O(2)-dependent epoxidation of styrene, cis-beta-methylstyrene, and cis-stilbene with retention of stereochemistry. Is able to use cumene hydroperoxide (CHP) or tert-butyl hydroperoxide (TBHP) instead of H(2)O(2) as the electron acceptor. Can also hydroxylate fatty acids such as lauric acid.<ref>PMID:10799487</ref> <ref>PMID:12010041</ref> <ref>PMID:18157853</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/1f4u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f4u ConSurf].
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<div style="clear:both"></div>
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'''THERMOPHILIC P450: CYP119 FROM SULFOLOBUS SOLFACTARICUS'''
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==See Also==
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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== References ==
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==Overview==
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<references/>
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The structure of the first P450 identified in Archaea, CYP119 from Sulfolobus solfataricus, has been solved in two different crystal forms that differ by the ligand (imidazole or 4-phenylimidazole) coordinated to the heme iron. A comparison of the two structures reveals an unprecedented rearrangement of the active site to adapt to the different size and shape of ligands bound to the heme iron. These changes involve unraveling of the F helix C-terminal segment to extend a loop structure connecting the F and G helices, allowing the longer loop to dip down into the active site and interact with the smaller imidazole ligand. A comparison of CYP119 with P450cam and P450eryF indicates an extensive clustering of aromatic residues may provide the structural basis for the enhanced thermal stability of CYP119. An additional feature of the 4-phenylimidazole-bound structure is a zinc ion tetrahedrally bound by symmetry-related His and Glu residues.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1F4U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4U OCA].
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[[Category: Saccharolobus solfataricus]]
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[[Category: Koo LS]]
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==Reference==
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[[Category: Li H]]
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Crystal structure of a thermophilic cytochrome P450 from the archaeon Sulfolobus solfataricus., Yano JK, Koo LS, Schuller DJ, Li H, Ortiz de Montellano PR, Poulos TL, J Biol Chem. 2000 Oct 6;275(40):31086-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10859321 10859321]
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[[Category: Ortiz de Montellano PR]]
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[[Category: Single protein]]
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[[Category: Poulos TL]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Schuller DJ]]
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[[Category: Koo, L S.]]
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[[Category: Yano JK]]
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[[Category: Li, H.]]
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[[Category: Montellano, P R.Ortiz de.]]
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[[Category: Poulos, T L.]]
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[[Category: Schuller, D J.]]
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[[Category: Yano, J K.]]
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[[Category: p450 fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:15:53 2008''
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Current revision

THERMOPHILIC P450: CYP119 FROM SULFOLOBUS SOLFACTARICUS

PDB ID 1f4u

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