1f6t

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[[Image:1f6t.jpg|left|200px]]
 
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{{Structure
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==STRUCTURE OF THE NUCLEOSIDE DIPHOSPHATE KINASE/ALPHA-BORANO(RP)-TDP.MG COMPLEX==
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|PDB= 1f6t |SIZE=350|CAPTION= <scene name='initialview01'>1f6t</scene>, resolution 1.92&Aring;
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<StructureSection load='1f6t' size='340' side='right'caption='[[1f6t]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TBD:2*-DEOXY-THYMIDINE-5*-ALPHA+BORANO+DIPHOSPHATE+(ISOMER+RP)'>TBD</scene>
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<table><tr><td colspan='2'>[[1f6t]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F6T FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TBD:2*-DEOXY-THYMIDINE-5*-ALPHA+BORANO+DIPHOSPHATE+(ISOMER+RP)'>TBD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6t OCA], [https://pdbe.org/1f6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f6t RCSB], [https://www.ebi.ac.uk/pdbsum/1f6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f6t ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1f3f|1F3F]], [[1lwx|1LWX]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6t OCA], [http://www.ebi.ac.uk/pdbsum/1f6t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f6t RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/NDKC_DICDI NDKC_DICDI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f6t ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF THE NUCLEOSIDE DIPHOSPHATE KINASE/ALPHA-BORANO(RP)-TDP.MG COMPLEX'''
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==See Also==
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*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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AIDS chemotherapy is limited by inadequate intracellular concentrations of the active triphosphate form of nucleoside analogues, leading to incomplete inhibition of viral replication and the appearance of drug-resistant virus. Drug activation by nucleoside diphosphate kinase and inhibition of HIV-1 reverse transcriptase were studied comparatively. We synthesized analogues with a borano (BH(3)(-)) group on the alpha-phosphate, and found that they are substrates for both enzymes. X-ray structures of complexes with nucleotide diphosphate kinase provided a structural basis for their activation. The complex with d4T triphosphate displayed an intramolecular CH.O bond contributing to catalysis, and the R(p) diastereoisomer of thymidine alpha-boranotriphosphate bound like a normal substrate. Using alpha-(R(p))-boranophosphate derivatives of the clinically relevant compounds AZT and d4T, the presence of the alpha-borano group improved both phosphorylation by nucleotide diphosphate kinase and inhibition of reverse transcription. Moreover, repair of blocked DNA chains by pyrophosphorolysis was reduced significantly in variant reverse transcriptases bearing substitutions found in drug-resistant viruses. Thus, the alpha-borano modification of analogues targeting reverse transcriptase may be of generic value in fighting viral drug resistance.
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==About this Structure==
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1F6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6T OCA].
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==Reference==
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Structural basis for activation of alpha-boranophosphate nucleotide analogues targeting drug-resistant reverse transcriptase., Meyer P, Schneider B, Sarfati S, Deville-Bonne D, Guerreiro C, Boretto J, Janin J, Veron M, Canard B, EMBO J. 2000 Jul 17;19(14):3520-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10899107 10899107]
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[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
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[[Category: Nucleoside-diphosphate kinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Boretto J]]
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[[Category: Boretto, J.]]
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[[Category: Canard B]]
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[[Category: Canard, B.]]
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[[Category: Deville-Bonne D]]
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[[Category: Deville-Bonne, D.]]
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[[Category: Guerreiro C]]
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[[Category: Guerreiro, C.]]
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[[Category: Janin J]]
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[[Category: Janin, J.]]
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[[Category: Sarfati S]]
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[[Category: Sarfati, S.]]
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[[Category: Schneider B]]
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[[Category: Schneider, B.]]
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[[Category: Veron M]]
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[[Category: Veron, M.]]
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[[Category: anti-hiv nucleoside analogue]]
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[[Category: boranophosphate]]
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[[Category: crystallography]]
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[[Category: nucleoside diphosphate kinase]]
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[[Category: phosphorylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:17:08 2008''
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Current revision

STRUCTURE OF THE NUCLEOSIDE DIPHOSPHATE KINASE/ALPHA-BORANO(RP)-TDP.MG COMPLEX

PDB ID 1f6t

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