1fa0

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[[Image:1fa0.jpg|left|200px]]
 
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{{Structure
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==STRUCTURE OF YEAST POLY(A) POLYMERASE BOUND TO MANGANATE AND 3'-DATP==
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|PDB= 1fa0 |SIZE=350|CAPTION= <scene name='initialview01'>1fa0</scene>, resolution 2.6&Aring;
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<StructureSection load='1fa0' size='340' side='right'caption='[[1fa0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=3AD:3&#39;-DEOXYADENOSINE'>3AD</scene>, <scene name='pdbligand=3AT:3&#39;-DEOXYADENOSINE-5&#39;-TRIPHOSPHATE'>3AT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>
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<table><tr><td colspan='2'>[[1fa0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The October 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Poly(A) Polymerase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_10 10.2210/rcsb_pdb/mom_2008_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FA0 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3AD:3-DEOXYADENOSINE'>3AD</scene>, <scene name='pdbligand=3AT:3-DEOXYADENOSINE-5-TRIPHOSPHATE'>3AT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fa0 OCA], [https://pdbe.org/1fa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fa0 RCSB], [https://www.ebi.ac.uk/pdbsum/1fa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fa0 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fa0 OCA], [http://www.ebi.ac.uk/pdbsum/1fa0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fa0 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PAP_YEAST PAP_YEAST] Polymerase component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.<ref>PMID:17850751</ref> <ref>PMID:18537269</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/1fa0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fa0 ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF YEAST POLY(A) POLYMERASE BOUND TO MANGANATE AND 3'-DATP'''
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==See Also==
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*[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Polyadenylate [poly(A)] polymerase (PAP) catalyzes the addition of a polyadenosine tail to almost all eukaryotic messenger RNAs (mRNAs). The crystal structure of the PAP from Saccharomyces cerevisiae (Pap1) has been solved to 2.6 angstroms, both alone and in complex with 3'-deoxyadenosine triphosphate (3'-dATP). Like other nucleic acid polymerases, Pap1 is composed of three domains that encircle the active site. The arrangement of these domains, however, is quite different from that seen in polymerases that use a template to select and position their incoming nucleotides. The first two domains are functionally analogous to polymerase palm and fingers domains. The third domain is attached to the fingers domain and is known to interact with the single-stranded RNA primer. In the nucleotide complex, two molecules of 3'-dATP are bound to Pap1. One occupies the position of the incoming base, prior to its addition to the mRNA chain. The other is believed to occupy the position of the 3' end of the mRNA primer.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1FA0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FA0 OCA].
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[[Category: RCSB PDB Molecule of the Month]]
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==Reference==
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Structure of yeast poly(A) polymerase alone and in complex with 3'-dATP., Bard J, Zhelkovsky AM, Helmling S, Earnest TN, Moore CL, Bohm A, Science. 2000 Aug 25;289(5483):1346-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10958780 10958780]
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[[Category: Polynucleotide adenylyltransferase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bard J]]
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[[Category: Bard, J.]]
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[[Category: Bohm A]]
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[[Category: Bohm, A.]]
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[[Category: Helmling S]]
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[[Category: Helmling, S.]]
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[[Category: Moore CL]]
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[[Category: Moore, C L.]]
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[[Category: Zhelkovsky AM]]
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[[Category: Zhelkovsky, A M.]]
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[[Category: nucleotidyl transferase]]
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[[Category: polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:18:58 2008''
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Current revision

STRUCTURE OF YEAST POLY(A) POLYMERASE BOUND TO MANGANATE AND 3'-DATP

PDB ID 1fa0

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