1fbw

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[[Image:1fbw.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the cellulase CEL48F from C. cellulolyticum in complex with cellohexaose==
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|PDB= 1fbw |SIZE=350|CAPTION= <scene name='initialview01'>1fbw</scene>, resolution 2.0&Aring;
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<StructureSection load='1fbw' size='340' side='right'caption='[[1fbw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>
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<table><tr><td colspan='2'>[[1fbw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminiclostridium_cellulolyticum Ruminiclostridium cellulolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FBW FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene>, <scene name='pdbligand=PRD_900035:alpha-maltohexaose'>PRD_900035</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fbw OCA], [https://pdbe.org/1fbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fbw RCSB], [https://www.ebi.ac.uk/pdbsum/1fbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fbw ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1fce|1FCE]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fbw OCA], [http://www.ebi.ac.uk/pdbsum/1fbw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fbw RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/GUNF_RUMCH GUNF_RUMCH] Probable endoglucanase involved in the degradation of cellulose or related beta-glucans.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/1fbw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fbw ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the cellulase CEL48F from C. cellulolyticum in complex with cellohexaose'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Cellulase Cel48F from Clostridium cellulolyticum was described as a processive endo-cellulase. The active site is composed of a 25 A long tunnel which is followed by an open cleft. During the processive action, the cellulose substrate has to slide through the tunnel to continuously supply the leaving group site with sugar residues after the catalytic cleavage. To study this processive action in the tunnel, the native catalytic module of Cel48F and the inactive mutant E55Q, have been cocrystallized with cellobiitol, two thio-oligosaccharide inhibitors (PIPS-IG3 and IG4) and the cello-oligosaccharides cellobiose, -tetraose and -hexaose. Seven sub-sites in the tunnel section of the active center could be identified and three of the four previously reported sub-sites in the open cleft section were reconfirmed. The sub-sites observed for the thio-oligosaccharide inhibitors and oligosaccharides, respectively, were located at two different positions in the tunnel corresponding to a shift in the chain direction of about a half sugar subunit. These two positions have different patterns of stacking interactions with aromatic residues present in the tunnel. Multiple patterns are not observed in nonprocessive endo-cellulases, where only one sugar position is favored by aromatic stacking. It is therefore proposed that the aromatic residues serve as lubricating agents to reduce the sliding barrier in the processive action.
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[[Category: Large Structures]]
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[[Category: Ruminiclostridium cellulolyticum]]
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==About this Structure==
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[[Category: Belaich JP]]
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1FBW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulolyticum Clostridium cellulolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBW OCA].
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[[Category: Driguez H]]
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[[Category: Haser R]]
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==Reference==
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[[Category: Parsiegla G]]
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Crystal structures of the cellulase Cel48F in complex with inhibitors and substrates give insights into its processive action., Parsiegla G, Reverbel-Leroy C, Tardif C, Belaich JP, Driguez H, Haser R, Biochemistry. 2000 Sep 19;39(37):11238-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10985769 10985769]
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[[Category: Reverbel-Leroy C]]
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[[Category: Cellulase]]
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[[Category: Tardif C]]
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[[Category: Clostridium cellulolyticum]]
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[[Category: Single protein]]
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[[Category: Belaich, J P.]]
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[[Category: Driguez, H.]]
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[[Category: Haser, R.]]
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[[Category: Parsiegla, G.]]
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[[Category: Reverbel-Leroy, C.]]
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[[Category: Tardif, C.]]
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[[Category: cellulase]]
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[[Category: protein-cellohexaose complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:01 2008''
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Current revision

Crystal structure of the cellulase CEL48F from C. cellulolyticum in complex with cellohexaose

PDB ID 1fbw

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